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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xylella fastidiosa 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP773152XAT
CSB-EP773152XAT
CSB-BP773152XAT
CSB-MP773152XAT
CSB-EP773152XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP773153XAT
CSB-EP773153XAT
CSB-BP773153XAT
CSB-MP773153XAT
CSB-EP773153XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP773154XAT
CSB-EP773154XAT
CSB-BP773154XAT
CSB-MP773154XAT
CSB-EP773154XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N-acetylmuramic acid 6-phosphate etherase 2 (murQ2) CSB-YP773155VFE
CSB-EP773155VFE
CSB-BP773155VFE
CSB-MP773155VFE
CSB-EP773155VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional regulator malT (malT), partial CSB-YP773156VFE
CSB-EP773156VFE
CSB-BP773156VFE
CSB-MP773156VFE
CSB-EP773156VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Multidrug resistance protein MdtL (mdtL), partial CSB-YP773157VFE
CSB-EP773157VFE
CSB-BP773157VFE
CSB-MP773157VFE
CSB-EP773157VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0397 protein VPA1481 (VPA1481), partial CSB-YP773158VFE1
CSB-EP773158VFE1
CSB-BP773158VFE1
CSB-MP773158VFE1
CSB-EP773158VFE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maltose transport system permease protein malG (malG), partial CSB-YP773159VFE1
CSB-EP773159VFE1
CSB-BP773159VFE1
CSB-MP773159VFE1
CSB-EP773159VFE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transaldolase (tal) CSB-YP773160VFE
CSB-EP773160VFE
CSB-BP773160VFE
CSB-MP773160VFE
CSB-EP773160VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribose import ATP-binding protein RbsA (rbsA), partial CSB-YP773161VFE
CSB-EP773161VFE
CSB-BP773161VFE
CSB-MP773161VFE
CSB-EP773161VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative reductase VPA0870 (VPA0870) CSB-YP773162VFE
CSB-EP773162VFE
CSB-BP773162VFE
CSB-MP773162VFE
CSB-EP773162VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta 2 (accD2) CSB-YP773163VFE
CSB-EP773163VFE
CSB-BP773163VFE
CSB-MP773163VFE
CSB-EP773163VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protoheme IX farnesyltransferase 2 (cyoE2), partial CSB-YP773164VFE
CSB-EP773164VFE
CSB-BP773164VFE
CSB-MP773164VFE
CSB-EP773164VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP773165VFE
CSB-EP773165VFE
CSB-BP773165VFE
CSB-MP773165VFE
CSB-EP773165VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flagellar P-ring protein 1 (flgI1) CSB-YP773166VFE
CSB-EP773166VFE
CSB-BP773166VFE
CSB-MP773166VFE
CSB-EP773166VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrolide export ATP-binding/permease protein MacB (macB), partial CSB-YP773167VFE1
CSB-EP773167VFE1
CSB-BP773167VFE1
CSB-MP773167VFE1
CSB-EP773167VFE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0345 protein VPA0057 (VPA0057) CSB-YP773168VFE
CSB-EP773168VFE
CSB-BP773168VFE
CSB-MP773168VFE
CSB-EP773168VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP773169VFE
CSB-EP773169VFE
CSB-BP773169VFE
CSB-MP773169VFE
CSB-EP773169VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional protein GlmU (glmU) CSB-YP773170VFE
CSB-EP773170VFE
CSB-BP773170VFE
CSB-MP773170VFE
CSB-EP773170VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP773171VFE
CSB-EP773171VFE
CSB-BP773171VFE
CSB-MP773171VFE
CSB-EP773171VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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