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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dictyostelium discoideum TNF receptor-associated factor family protein DDB_G0272340 (DDB_G0272340) CSB-YP772972DKK
CSB-EP772972DKK
CSB-BP772972DKK
CSB-MP772972DKK
CSB-EP772972DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Probable ATP-dependent RNA helicase CG8611 (CG8611), partial CSB-YP772973DLU
CSB-EP772973DLU
CSB-BP772973DLU
CSB-MP772973DLU
CSB-EP772973DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein-methionine sulfoxide oxidase Mical (Mical), partial CSB-YP772974DLU
CSB-EP772974DLU
CSB-BP772974DLU
CSB-MP772974DLU
CSB-EP772974DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Methyltransferase-like protein (metl) CSB-YP772975DLU
CSB-EP772975DLU
CSB-BP772975DLU
CSB-MP772975DLU
CSB-EP772975DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Ubiquitin-like modifier-activating enzyme atg7 (atg7), partial CSB-YP772976DKK
CSB-EP772976DKK
CSB-BP772976DKK
CSB-MP772976DKK
CSB-EP772976DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein C05B5.8 (C05B5.8), partial CSB-YP772977CXY1
CSB-EP772977CXY1
CSB-BP772977CXY1
CSB-MP772977CXY1
CSB-EP772977CXY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sagartia rosea Cytolysin Src-1 CSB-YP772978SHAU
CSB-EP772978SHAU
CSB-BP772978SHAU
CSB-MP772978SHAU
CSB-EP772978SHAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pandalus borealis Crustapain (Cys) CSB-YP772979PAT
CSB-EP772979PAT
CSB-BP772979PAT
CSB-MP772979PAT
CSB-EP772979PAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0275657 (DDB_G0275657) CSB-YP772980DKK
CSB-EP772980DKK
CSB-BP772980DKK
CSB-MP772980DKK
CSB-EP772980DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Protein Asterix (DDB_G0275849), partial CSB-YP772981DKK1
CSB-EP772981DKK1
CSB-BP772981DKK1
CSB-MP772981DKK1
CSB-EP772981DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0275565 (DDB_G0275565) CSB-YP772982DKK
CSB-EP772982DKK
CSB-BP772982DKK
CSB-MP772982DKK
CSB-EP772982DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Counting factor 45-1 (cf45-1) CSB-YP772983DKK
CSB-EP772983DKK
CSB-BP772983DKK
CSB-MP772983DKK
CSB-EP772983DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Metabotropic glutamate receptor-like protein B (grlB), partial CSB-YP772984DKK1
CSB-EP772984DKK1
CSB-BP772984DKK1
CSB-MP772984DKK1
CSB-EP772984DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum SPX and EXS domain-containing protein 2 (DDB_G0274481), partial CSB-YP772985DKK
CSB-EP772985DKK
CSB-BP772985DKK
CSB-MP772985DKK
CSB-EP772985DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative mediator of RNA polymerase II transcription subunit 30 (med30) CSB-YP772986DKK
CSB-EP772986DKK
CSB-BP772986DKK
CSB-MP772986DKK
CSB-EP772986DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0276433 (DDB_G0276433) CSB-YP772987DKK
CSB-EP772987DKK
CSB-BP772987DKK
CSB-MP772987DKK
CSB-EP772987DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable serine/threonine-protein kinase nek3 (nek3), partial CSB-YP772988DKK
CSB-EP772988DKK
CSB-BP772988DKK
CSB-MP772988DKK
CSB-EP772988DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum O-methyltransferase 4 (omt4) CSB-YP772989DKK
CSB-EP772989DKK
CSB-BP772989DKK
CSB-MP772989DKK
CSB-EP772989DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum UPF0519 protein B (sigN122) CSB-YP772990DKK
CSB-EP772990DKK
CSB-BP772990DKK
CSB-MP772990DKK
CSB-EP772990DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable caffeoyl-CoA O-methyltransferase 1 (omt5) CSB-YP772991DKK
CSB-EP772991DKK
CSB-BP772991DKK
CSB-MP772991DKK
CSB-EP772991DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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