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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Emericella nidulans Siderophore iron transporter mirC (mirC), partial CSB-YP773072EKF
CSB-EP773072EKF
CSB-BP773072EKF
CSB-MP773072EKF
CSB-EP773072EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Mannose-6-phosphate isomerase (103E1.170, NCU07165) CSB-YP773073NHA
CSB-EP773073NHA
CSB-BP773073NHA
CSB-MP773073NHA
CSB-EP773073NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Ubiquitin-like modifier-activating enzyme atg-7 (atg-7), partial CSB-YP773074NHA
CSB-EP773074NHA
CSB-BP773074NHA
CSB-MP773074NHA
CSB-EP773074NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Autophagy protein 5 (atg-5) CSB-YP773075NHA
CSB-EP773075NHA
CSB-BP773075NHA
CSB-MP773075NHA
CSB-EP773075NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Shugoshin (sgo-1), partial CSB-YP773076NHA
CSB-EP773076NHA
CSB-BP773076NHA
CSB-MP773076NHA
CSB-EP773076NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata 40S ribosomal protein S23 (rps23) CSB-YP773077NGS
CSB-EP773077NGS
CSB-BP773077NGS
CSB-MP773077NGS
CSB-EP773077NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pichia pastoris Elongation factor 2 (EFT1), partial CSB-YP773078EVS
CSB-EP773078EVS
CSB-BP773078EVS
CSB-MP773078EVS
CSB-EP773078EVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans 3-keto-steroid reductase (ERG27) CSB-YP773079CZF
CSB-EP773079CZF
CSB-BP773079CZF
CSB-MP773079CZF
CSB-EP773079CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Putative cell agglutination protein C947.04 (SPBC947.04), partial CSB-YP773080SXV
CSB-EP773080SXV
CSB-BP773080SXV
CSB-MP773080SXV
CSB-EP773080SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Autophagy-related protein 9 (ATG9), partial CSB-YP773081EXI
CSB-EP773081EXI
CSB-BP773081EXI
CSB-MP773081EXI
CSB-EP773081EXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryphonectria parasitica Mitogen-activated protein kinase HOG1 (HOG1) CSB-YP773082DYH
CSB-EP773082DYH
CSB-BP773082DYH
CSB-MP773082DYH
CSB-EP773082DYH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea kluyveri Heat shock protein homolog SSE1 (SSE1), partial CSB-YP773083LMA
CSB-EP773083LMA
CSB-BP773083LMA
CSB-MP773083LMA
CSB-EP773083LMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea kluyveri Pyruvate kinase (PYK1), partial CSB-YP773084LMA
CSB-EP773084LMA
CSB-BP773084LMA
CSB-MP773084LMA
CSB-EP773084LMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naumovozyma castellii Myosin-2B (MYO2B), partial CSB-YP773085NAY
CSB-EP773085NAY
CSB-BP773085NAY
CSB-MP773085NAY
CSB-EP773085NAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces bayanus Protein transport protein SEC24-2 (SEC242), partial CSB-YP773086SVF
CSB-EP773086SVF
CSB-BP773086SVF
CSB-MP773086SVF
CSB-EP773086SVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Ubiquitination network signaling protein acrB (acrB), partial CSB-YP773087EKF
CSB-EP773087EKF
CSB-BP773087EKF
CSB-MP773087EKF
CSB-EP773087EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus kodakaraensis Deoxyribose-phosphate aldolase (deoC) CSB-YP773088FHY
CSB-EP773088FHY
CSB-BP773088FHY
CSB-MP773088FHY
CSB-EP773088FHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa tRNA modification GTPase MnmE (mnmE) CSB-YP773089XAT
CSB-EP773089XAT
CSB-BP773089XAT
CSB-MP773089XAT
CSB-EP773089XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Glutamyl-tRNA reductase (hemA) CSB-YP773090XAT
CSB-EP773090XAT
CSB-BP773090XAT
CSB-MP773090XAT
CSB-EP773090XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L1 (rplA) CSB-YP773091XAT
CSB-EP773091XAT
CSB-BP773091XAT
CSB-MP773091XAT
CSB-EP773091XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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