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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xylella fastidiosa Shikimate kinase (aroK) CSB-YP773132XAT
CSB-EP773132XAT
CSB-BP773132XAT
CSB-MP773132XAT
CSB-EP773132XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Enolase (eno) CSB-YP773133XAT
CSB-EP773133XAT
CSB-BP773133XAT
CSB-MP773133XAT
CSB-EP773133XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Putative phosphotransferase PD_0525 (PD_0525) CSB-YP773134XAT
CSB-EP773134XAT
CSB-BP773134XAT
CSB-MP773134XAT
CSB-EP773134XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Lipase chaperone (lifO), partial CSB-YP773135XAT1
CSB-EP773135XAT1
CSB-BP773135XAT1
CSB-MP773135XAT1
CSB-EP773135XAT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L30 (rpmD) CSB-YP773136XAT
CSB-EP773136XAT
CSB-BP773136XAT
CSB-MP773136XAT
CSB-EP773136XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S8 (rpsH) CSB-YP773137XAT
CSB-EP773137XAT
CSB-BP773137XAT
CSB-MP773137XAT
CSB-EP773137XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S19 (rpsS) CSB-YP773138XAT
CSB-EP773138XAT
CSB-BP773138XAT
CSB-MP773138XAT
CSB-EP773138XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa ATP synthase subunit b (atpF), partial CSB-YP773139XAT
CSB-EP773139XAT
CSB-BP773139XAT
CSB-MP773139XAT
CSB-EP773139XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Bifunctional protein GlmU (glmU) CSB-YP773140XAT
CSB-EP773140XAT
CSB-BP773140XAT
CSB-MP773140XAT
CSB-EP773140XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP773141XAT
CSB-EP773141XAT
CSB-BP773141XAT
CSB-MP773141XAT
CSB-EP773141XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP773142XAT
CSB-EP773142XAT
CSB-BP773142XAT
CSB-MP773142XAT
CSB-EP773142XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribosome-recycling factor (frr) CSB-YP773143XAT
CSB-EP773143XAT
CSB-BP773143XAT
CSB-MP773143XAT
CSB-EP773143XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP773144XAT
CSB-EP773144XAT
CSB-BP773144XAT
CSB-MP773144XAT
CSB-EP773144XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Gamma-glutamyl phosphate reductase (proA) CSB-YP773145XAT
CSB-EP773145XAT
CSB-BP773145XAT
CSB-MP773145XAT
CSB-EP773145XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP773146XAT1
CSB-EP773146XAT1
CSB-BP773146XAT1
CSB-MP773146XAT1
CSB-EP773146XAT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S15 (rpsO) CSB-YP773147XAT
CSB-EP773147XAT
CSB-BP773147XAT
CSB-MP773147XAT
CSB-EP773147XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Glucose-6-phosphate isomerase (pgi), partial CSB-YP773148XAT
CSB-EP773148XAT
CSB-BP773148XAT
CSB-MP773148XAT
CSB-EP773148XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP773149XAT
CSB-EP773149XAT
CSB-BP773149XAT
CSB-MP773149XAT
CSB-EP773149XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UPF0758 protein PD_0117 (PD_0117) CSB-YP773150XAT
CSB-EP773150XAT
CSB-BP773150XAT
CSB-MP773150XAT
CSB-EP773150XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Probable cytosol aminopeptidase (pepA) CSB-YP773151XAT
CSB-EP773151XAT
CSB-BP773151XAT
CSB-MP773151XAT
CSB-EP773151XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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