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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica Probable aldehyde oxidase 1 (Os10g0138100, LOC_Os10g04860), partial CSB-YP800022OFG
CSB-EP800022OFG
CSB-BP800022OFG
CSB-MP800022OFG
CSB-EP800022OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable calcium-binding protein CML24 (CML24) CSB-YP800023OFG
CSB-EP800023OFG
CSB-BP800023OFG
CSB-MP800023OFG
CSB-EP800023OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein YABBY 1 (YAB1) CSB-YP800024OFG
CSB-EP800024OFG
CSB-BP800024OFG
CSB-MP800024OFG
CSB-EP800024OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Metacaspase-2 (AMC2) CSB-YP800025DOA
CSB-EP800025DOA
CSB-BP800025DOA
CSB-MP800025DOA
CSB-EP800025DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Scarecrow-like protein 27 (SCL27), partial CSB-YP800026DOA
CSB-EP800026DOA
CSB-BP800026DOA
CSB-MP800026DOA
CSB-EP800026DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Pachytene checkpoint protein 2 homolog (Os04g0479000, LOC_Os04g40290) CSB-YP800027OFG
CSB-EP800027OFG
CSB-BP800027OFG
CSB-MP800027OFG
CSB-EP800027OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Beta-glucosidase 13 (BGLU13) CSB-YP800028OFG
CSB-EP800028OFG
CSB-BP800028OFG
CSB-MP800028OFG
CSB-EP800028OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica DNA-binding protein S1FA2 (S1FA2) CSB-YP800029OFG
CSB-EP800029OFG
CSB-BP800029OFG
CSB-MP800029OFG
CSB-EP800029OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable ascorbate-specific transmembrane electron transporter 2 (Os04g0533500, LOC_Os04g45090), partial CSB-YP800030OFG1
CSB-EP800030OFG1
CSB-BP800030OFG1
CSB-MP800030OFG1
CSB-EP800030OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable cation transporter HKT7 (HKT7), partial CSB-YP800031OFG
CSB-EP800031OFG
CSB-BP800031OFG
CSB-MP800031OFG
CSB-EP800031OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Peroxisomal (S)-2-hydroxy-acid oxidase GLO2 (GLO2) CSB-YP800032OFG
CSB-EP800032OFG
CSB-BP800032OFG
CSB-MP800032OFG
CSB-EP800032OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable inactive beta-glucosidase 14 (BGLU14) CSB-YP800033OFG
CSB-EP800033OFG
CSB-BP800033OFG
CSB-MP800033OFG
CSB-EP800033OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein phosphatase 2C 45 (Os04g0659500, LOC_Os04g56450) CSB-YP800034OFG
CSB-EP800034OFG
CSB-BP800034OFG
CSB-MP800034OFG
CSB-EP800034OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein disulfide isomerase-like 1-2 (PDIL1-2) CSB-YP800035OFG
CSB-EP800035OFG
CSB-BP800035OFG
CSB-MP800035OFG
CSB-EP800035OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Beta-glucosidase 18 (BGLU18) CSB-YP800036OFG
CSB-EP800036OFG
CSB-BP800036OFG
CSB-MP800036OFG
CSB-EP800036OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Auxin response factor 13 (ARF13), partial CSB-YP800037OFG
CSB-EP800037OFG
CSB-BP800037OFG
CSB-MP800037OFG
CSB-EP800037OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Squamosa promoter-binding-like protein 7 (SPL7) CSB-YP800038OFG
CSB-EP800038OFG
CSB-BP800038OFG
CSB-MP800038OFG
CSB-EP800038OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Vacuolar iron transporter homolog 5 (Os04g0686800, LOC_Os04g59020), partial CSB-YP800039OFG1
CSB-EP800039OFG1
CSB-BP800039OFG1
CSB-MP800039OFG1
CSB-EP800039OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein phosphatase 2C 43 (Os04g0584300, Os04g0584366, LOC_Os04g49490) CSB-YP800040OFG
CSB-EP800040OFG
CSB-BP800040OFG
CSB-MP800040OFG
CSB-EP800040OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Peptide methionine sulfoxide reductase A2-2 (MSRA2-2) CSB-YP800041OFG
CSB-EP800041OFG
CSB-BP800041OFG
CSB-MP800041OFG
CSB-EP800041OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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