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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP774491SZB
CSB-EP774491SZB
CSB-BP774491SZB
CSB-MP774491SZB
CSB-EP774491SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c biogenesis ATP-binding export protein CcmA (ccmA) CSB-YP774492SZB
CSB-EP774492SZB
CSB-BP774492SZB
CSB-MP774492SZB
CSB-EP774492SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydroxyethylthiazole kinase (thiM) CSB-YP774493SZB
CSB-EP774493SZB
CSB-BP774493SZB
CSB-MP774493SZB
CSB-EP774493SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative multidrug resistance protein MdtA (mdtA) CSB-YP774494SZB
CSB-BP774494SZB
CSB-MP774494SZB
CSB-EP774494SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein mtfA (mtfA) CSB-YP774495SZB
CSB-EP774495SZB
CSB-BP774495SZB
CSB-MP774495SZB
CSB-EP774495SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP774496SZB
CSB-EP774496SZB
CSB-BP774496SZB
CSB-MP774496SZB
CSB-EP774496SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Diguanylate cyclase yddV (yddV) CSB-YP774497SZB
CSB-EP774497SZB
CSB-BP774497SZB
CSB-MP774497SZB
CSB-EP774497SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannose-6-phosphate isomerase (manA) CSB-YP774498SZB
CSB-EP774498SZB
CSB-BP774498SZB
CSB-MP774498SZB
CSB-EP774498SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP774499SZB
CSB-EP774499SZB
CSB-BP774499SZB
CSB-MP774499SZB
CSB-EP774499SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP774500SZB
CSB-EP774500SZB
CSB-BP774500SZB
CSB-MP774500SZB
CSB-EP774500SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0509 protein yciZ (yciZ) CSB-YP774501SZB
CSB-EP774501SZB
CSB-BP774501SZB
CSB-MP774501SZB
CSB-EP774501SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant High frequency lysogenization protein HflD homolog (hflD) CSB-YP774502SZB
CSB-EP774502SZB
CSB-BP774502SZB
CSB-MP774502SZB
CSB-EP774502SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deferrochelatase/peroxidase EfeB (efeB) CSB-YP774503SZB
CSB-EP774503SZB
CSB-BP774503SZB
CSB-MP774503SZB
CSB-EP774503SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP774504SZB
CSB-EP774504SZB
CSB-BP774504SZB
CSB-MP774504SZB
CSB-EP774504SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0145 protein YbjQ (ybjQ) CSB-YP774505SZB
CSB-EP774505SZB
CSB-BP774505SZB
CSB-MP774505SZB
CSB-EP774505SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipid A palmitoyltransferase PagP (pagP) CSB-YP774506SZB
CSB-EP774506SZB
CSB-BP774506SZB
CSB-MP774506SZB
CSB-EP774506SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endonuclease 8 (nei) CSB-YP774507SZB
CSB-EP774507SZB
CSB-BP774507SZB
CSB-MP774507SZB
CSB-EP774507SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Taurine import ATP-binding protein TauB (tauB) CSB-YP774508SZB
CSB-EP774508SZB
CSB-BP774508SZB
CSB-MP774508SZB
CSB-EP774508SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deoxyguanosinetriphosphate triphosphohydrolase (dgt), partial CSB-YP774509SZB
CSB-EP774509SZB
CSB-BP774509SZB
CSB-MP774509SZB
CSB-EP774509SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP774510SZB
CSB-EP774510SZB
CSB-BP774510SZB
CSB-MP774510SZB
CSB-EP774510SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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