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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xylella fastidiosa 30S ribosomal protein S17 (rpsQ) CSB-YP774913XAT
CSB-EP774913XAT
CSB-BP774913XAT
CSB-MP774913XAT
CSB-EP774913XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L23 (rplW) CSB-YP774914XAT
CSB-EP774914XAT
CSB-BP774914XAT
CSB-MP774914XAT
CSB-EP774914XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa ATP synthase subunit beta (atpD) CSB-YP774915XAT
CSB-EP774915XAT
CSB-BP774915XAT
CSB-MP774915XAT
CSB-EP774915XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Lysine--tRNA ligase (lysS), partial CSB-YP774916XAT
CSB-EP774916XAT
CSB-BP774916XAT
CSB-MP774916XAT
CSB-EP774916XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Lipoprotein-releasing system transmembrane protein LolC (lolC), partial CSB-YP774917XAT1
CSB-EP774917XAT1
CSB-BP774917XAT1
CSB-MP774917XAT1
CSB-EP774917XAT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Undecaprenyl pyrophosphate synthase (uppS) CSB-YP774918XAT
CSB-EP774918XAT
CSB-BP774918XAT
CSB-MP774918XAT
CSB-EP774918XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Adenosylhomocysteinase (ahcY) CSB-YP774919XAT
CSB-EP774919XAT
CSB-BP774919XAT
CSB-MP774919XAT
CSB-EP774919XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Cysteine--tRNA ligase (cysS) CSB-YP774920XAT
CSB-EP774920XAT
CSB-BP774920XAT
CSB-MP774920XAT
CSB-EP774920XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP774921XAT
CSB-EP774921XAT
CSB-BP774921XAT
CSB-MP774921XAT
CSB-EP774921XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa tRNA pseudouridine synthase B (truB) CSB-YP774922XAT
CSB-EP774922XAT
CSB-BP774922XAT
CSB-MP774922XAT
CSB-EP774922XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP774923XAT
CSB-EP774923XAT
CSB-BP774923XAT
CSB-MP774923XAT
CSB-EP774923XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa DNA polymerase III subunit alpha (dnaE), partial CSB-YP774924XAT
CSB-EP774924XAT
CSB-BP774924XAT
CSB-MP774924XAT
CSB-EP774924XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP774925XAT
CSB-EP774925XAT
CSB-BP774925XAT
CSB-MP774925XAT
CSB-EP774925XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP774926XAT
CSB-EP774926XAT
CSB-BP774926XAT
CSB-MP774926XAT
CSB-EP774926XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP774927XAT
CSB-EP774927XAT
CSB-BP774927XAT
CSB-MP774927XAT
CSB-EP774927XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Protein hfq (hfq) CSB-YP774928XAT
CSB-EP774928XAT
CSB-BP774928XAT
CSB-MP774928XAT
CSB-EP774928XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa DNA replication and repair protein recF (recF) CSB-YP774929XAT
CSB-EP774929XAT
CSB-BP774929XAT
CSB-MP774929XAT
CSB-EP774929XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannonate dehydratase (uxuA) CSB-YP774930VFE
CSB-EP774930VFE
CSB-BP774930VFE
CSB-MP774930VFE
CSB-EP774930VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP774931VFE
CSB-EP774931VFE
CSB-BP774931VFE
CSB-MP774931VFE
CSB-EP774931VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative ABC transporter ATP-binding protein VPA1482 (VPA1482), partial CSB-YP774932VFE
CSB-EP774932VFE
CSB-BP774932VFE
CSB-MP774932VFE
CSB-EP774932VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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