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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Calycanthus floridus var. glaucus Apocytochrome f (petA), partial CSB-YP800062CBM1
CSB-EP800062CBM1
CSB-BP800062CBM1
CSB-MP800062CBM1
CSB-EP800062CBM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calycanthus floridus var. glaucus NAD (P)H-quinone oxidoreductase subunit J, chloroplastic (ndhJ) CSB-YP800063CBM
CSB-EP800063CBM
CSB-BP800063CBM
CSB-MP800063CBM
CSB-EP800063CBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calycanthus floridus var. glaucus Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP800064CBM1
CSB-EP800064CBM1
CSB-BP800064CBM1
CSB-MP800064CBM1
CSB-EP800064CBM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calycanthus floridus var. glaucus ATP synthase subunit alpha, chloroplastic (atpA), partial CSB-YP800065CBM
CSB-EP800065CBM
CSB-BP800065CBM
CSB-MP800065CBM
CSB-EP800065CBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gratiola officinalis Maturase K (matK), partial CSB-YP800066GCAU
CSB-EP800066GCAU
CSB-BP800066GCAU
CSB-MP800066GCAU
CSB-EP800066GCAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Galbulimima belgraveana Maturase K (matK), partial CSB-YP800067GCAV
CSB-EP800067GCAV
CSB-BP800067GCAV
CSB-MP800067GCAV
CSB-EP800067GCAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clematis lasiantha Maturase K (matK), partial CSB-YP800068CJAH
CSB-EP800068CJAH
CSB-BP800068CJAH
CSB-MP800068CJAH
CSB-EP800068CJAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eimeria tenella 50S ribosomal protein L2, apicoplast (rpl2) CSB-YP800069EJU
CSB-EP800069EJU
CSB-BP800069EJU
CSB-MP800069EJU
CSB-EP800069EJU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Suaeda maritima Protein psbN (psbN), partial CSB-YP800070SHAV
CSB-EP800070SHAV
CSB-BP800070SHAV
CSB-MP800070SHAV
CSB-EP800070SHAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Heat shock 70 kDa protein 1 (HSPA1), partial CSB-YP800071DO
CSB-EP800071DO
CSB-BP800071DO
CSB-MP800071DO
CSB-EP800071DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit 4F2 cell-surface antigen heavy chain (SLC3A2), partial CSB-YP800072RB
CSB-BP800072RB
CSB-MP800072RB
CSB-EP800072RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Oligophrenin-1 (OPHN1), partial CSB-YP800073EQV
CSB-EP800073EQV
CSB-BP800073EQV
CSB-MP800073EQV
CSB-EP800073EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes DNA dC->dU-editing enzyme APOBEC-3G (APOBEC3G) CSB-YP800074EQV
CSB-EP800074EQV
CSB-BP800074EQV
CSB-MP800074EQV
CSB-EP800074EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Serine/threonine-protein phosphatase 1 regulatory subunit 10 (PPP1R10), partial CSB-YP800075EQV
CSB-EP800075EQV
CSB-BP800075EQV
CSB-MP800075EQV
CSB-EP800075EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Psoriasis susceptibility 1 candidate gene 2 protein homolog (PSORS1C2) CSB-YP800076EQV
CSB-EP800076EQV
CSB-BP800076EQV
CSB-MP800076EQV
CSB-EP800076EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine AH receptor-interacting protein (AIP) CSB-YP800077BO
CSB-EP800077BO
CSB-BP800077BO
CSB-MP800077BO
CSB-EP800077BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cebus capucinus Ribonuclease-like protein 9 (RNASE9) CSB-YP800078CFK
CSB-EP800078CFK
CSB-BP800078CFK
CSB-MP800078CFK
CSB-EP800078CFK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Transcription factor SOX-9 (SOX9), partial CSB-YP800079DO
CSB-EP800079DO
CSB-BP800079DO
CSB-MP800079DO
CSB-EP800079DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tarsius syrichta Cytochrome c oxidase subunit 6B1 (COX6B1) CSB-YP800080TLJ
CSB-EP800080TLJ
CSB-BP800080TLJ
CSB-MP800080TLJ
CSB-EP800080TLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Vomeronasal type-1 receptor 3 (VN1R3), partial CSB-YP800081EQV1
CSB-EP800081EQV1
CSB-BP800081EQV1
CSB-MP800081EQV1
CSB-EP800081EQV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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