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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterococcus faecalis UPF0234 protein EF_1165 (EF_1165) CSB-YP802674ELW
CSB-EP802674ELW
CSB-BP802674ELW
CSB-MP802674ELW
CSB-EP802674ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP802675ELW
CSB-EP802675ELW
CSB-BP802675ELW
CSB-MP802675ELW
CSB-EP802675ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Galactokinase (galK) CSB-YP802676ELW
CSB-EP802676ELW
CSB-BP802676ELW
CSB-MP802676ELW
CSB-EP802676ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP802677ELW
CSB-EP802677ELW
CSB-BP802677ELW
CSB-MP802677ELW
CSB-EP802677ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 50S ribosomal protein L21 (rplU) CSB-YP802678ELW
CSB-EP802678ELW
CSB-BP802678ELW
CSB-MP802678ELW
CSB-EP802678ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Putative sugar uptake protein EF_0928 (EF_0928), partial CSB-YP802679ELW
CSB-EP802679ELW
CSB-BP802679ELW
CSB-MP802679ELW
CSB-EP802679ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP802680ELW
CSB-EP802680ELW
CSB-BP802680ELW
CSB-MP802680ELW
CSB-EP802680ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Leucine--tRNA ligase (leuS), partial CSB-YP802681ELW
CSB-EP802681ELW
CSB-BP802681ELW
CSB-MP802681ELW
CSB-EP802681ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Putative agmatine deiminase (aguA) CSB-YP802682ELW
CSB-EP802682ELW
CSB-BP802682ELW
CSB-MP802682ELW
CSB-EP802682ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Tagatose 1,6-diphosphate aldolase 1 (lacD1) CSB-YP802683ELW
CSB-EP802683ELW
CSB-BP802683ELW
CSB-MP802683ELW
CSB-EP802683ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Norwalk virus Genome polyprotein (ORF1), partial CSB-YP802684NBAY1
CSB-EP802684NBAY1
CSB-BP802684NBAY1
CSB-MP802684NBAY1
CSB-EP802684NBAY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Diaminopimelate epimerase (dapF) CSB-YP802685ELW
CSB-EP802685ELW
CSB-BP802685ELW
CSB-MP802685ELW
CSB-EP802685ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase 1 (kduI1) CSB-YP802686ELW
CSB-EP802686ELW
CSB-BP802686ELW
CSB-MP802686ELW
CSB-EP802686ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oat chlorotic stunt virus Capsid protein (ORF2) CSB-YP802687OBAK
CSB-EP802687OBAK
CSB-BP802687OBAK
CSB-MP802687OBAK
CSB-EP802687OBAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orgyia pseudotsugata multicapsid polyhedrosis virus Occlusion-derived virus envelope protein E56 (ODVP6E), partial CSB-YP802688OEU1
CSB-EP802688OEU1
CSB-BP802688OEU1
CSB-MP802688OEU1
CSB-EP802688OEU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Hypoxanthine-guanine phosphoribosyltransferase (hpt) CSB-YP802689ELW
CSB-EP802689ELW
CSB-BP802689ELW
CSB-MP802689ELW
CSB-EP802689ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 50S ribosomal protein L17 (rplQ) CSB-YP802690ELW
CSB-EP802690ELW
CSB-BP802690ELW
CSB-MP802690ELW
CSB-EP802690ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Translation initiation factor IF-1 (infA) CSB-YP802691ELW
CSB-EP802691ELW
CSB-BP802691ELW
CSB-MP802691ELW
CSB-EP802691ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 30S ribosomal protein S14 type Z (rpsZ) CSB-YP802692ELW
CSB-EP802692ELW
CSB-BP802692ELW
CSB-MP802692ELW
CSB-EP802692ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 50S ribosomal protein L22 (rplV) CSB-YP802693ELW
CSB-EP802693ELW
CSB-BP802693ELW
CSB-MP802693ELW
CSB-EP802693ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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