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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Type II pantothenate kinase (coaW) CSB-YP802334BAM
CSB-EP802334BAM
CSB-BP802334BAM
CSB-MP802334BAM
CSB-EP802334BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Kynureninase (kynU) CSB-YP802335BAM
CSB-EP802335BAM
CSB-BP802335BAM
CSB-MP802335BAM
CSB-EP802335BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Stage II sporulation protein SB (spoIISB) CSB-YP802336BAM
CSB-EP802336BAM
CSB-BP802336BAM
CSB-MP802336BAM
CSB-EP802336BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methylmalonate semialdehyde dehydrogenase [acylating] (iolA) CSB-YP802337BAM
CSB-EP802337BAM
CSB-BP802337BAM
CSB-MP802337BAM
CSB-EP802337BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0403 protein BC_2157 (BC_2157) CSB-YP802338BAM
CSB-EP802338BAM
CSB-BP802338BAM
CSB-MP802338BAM
CSB-EP802338BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Uncharacterized HTH-type transcriptional regulator BC_1936 (BC_1936) CSB-YP802339BAM
CSB-EP802339BAM
CSB-BP802339BAM
CSB-MP802339BAM
CSB-EP802339BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP802340BAM
CSB-EP802340BAM
CSB-BP802340BAM
CSB-MP802340BAM
CSB-EP802340BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein H 2 (sspH2) CSB-YP802341BAM
CSB-EP802341BAM
CSB-BP802341BAM
CSB-MP802341BAM
CSB-EP802341BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydrodipicolinate reductase (dapB) CSB-YP802342BAM
CSB-EP802342BAM
CSB-BP802342BAM
CSB-MP802342BAM
CSB-EP802342BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Sulfate adenylyltransferase (sat) CSB-YP802343BAM
CSB-EP802343BAM
CSB-BP802343BAM
CSB-MP802343BAM
CSB-EP802343BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP802344BAM
CSB-EP802344BAM
CSB-BP802344BAM
CSB-MP802344BAM
CSB-EP802344BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-isopropylmalate dehydrogenase (leuB) CSB-YP802345BAM
CSB-EP802345BAM
CSB-BP802345BAM
CSB-MP802345BAM
CSB-EP802345BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP802346BAM
CSB-EP802346BAM
CSB-BP802346BAM
CSB-MP802346BAM
CSB-EP802346BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-aminoethylphosphonate--pyruvate transaminase (phnW) CSB-YP802347BAM
CSB-EP802347BAM
CSB-BP802347BAM
CSB-MP802347BAM
CSB-EP802347BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 2-oxoglutarate dehydrogenase E1 component (odhA), partial CSB-YP802348BAM
CSB-EP802348BAM
CSB-BP802348BAM
CSB-MP802348BAM
CSB-EP802348BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Bis (5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] (prpE) CSB-YP802349BAM
CSB-EP802349BAM
CSB-BP802349BAM
CSB-MP802349BAM
CSB-EP802349BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0344 protein BC_1150 (BC_1150), partial CSB-YP802350BAM1
CSB-EP802350BAM1
CSB-BP802350BAM1
CSB-MP802350BAM1
CSB-EP802350BAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ferrochelatase 1 (hemH1) CSB-YP802351BAM
CSB-EP802351BAM
CSB-BP802351BAM
CSB-MP802351BAM
CSB-EP802351BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BC_0885) CSB-YP802352BAM
CSB-EP802352BAM
CSB-BP802352BAM
CSB-MP802352BAM
CSB-EP802352BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Quinol oxidase subunit 2 (qoxA), partial CSB-YP802353BAM1
CSB-EP802353BAM1
CSB-BP802353BAM1
CSB-MP802353BAM1
CSB-EP802353BAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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