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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas putida 50S ribosomal protein L31 (rpmE) CSB-YP803455FGB
CSB-EP803455FGB
CSB-BP803455FGB
CSB-MP803455FGB
CSB-EP803455FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Urocanate hydratase (hutU), partial CSB-YP803456FGB
CSB-EP803456FGB
CSB-BP803456FGB
CSB-MP803456FGB
CSB-EP803456FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP803457FGB
CSB-EP803457FGB
CSB-BP803457FGB
CSB-MP803457FGB
CSB-EP803457FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida ATP-dependent protease subunit HslV (hslV) CSB-YP803458FGB
CSB-EP803458FGB
CSB-BP803458FGB
CSB-MP803458FGB
CSB-EP803458FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Phosphoglycerate kinase (pgk) CSB-YP803459FGB
CSB-EP803459FGB
CSB-BP803459FGB
CSB-MP803459FGB
CSB-EP803459FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Protein hfq (hfq) CSB-YP803460FGB
CSB-EP803460FGB
CSB-BP803460FGB
CSB-MP803460FGB
CSB-EP803460FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 30S ribosomal protein S18 (rpsR) CSB-YP803461FGB
CSB-EP803461FGB
CSB-BP803461FGB
CSB-MP803461FGB
CSB-EP803461FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP803462FGB
CSB-EP803462FGB
CSB-BP803462FGB
CSB-MP803462FGB
CSB-EP803462FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Gamma-glutamyl phosphate reductase (proA) CSB-YP803463FGB
CSB-EP803463FGB
CSB-BP803463FGB
CSB-MP803463FGB
CSB-EP803463FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Apolipoprotein N-acyltransferase (lnt), partial CSB-YP803464FGB1
CSB-EP803464FGB1
CSB-BP803464FGB1
CSB-MP803464FGB1
CSB-EP803464FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Chaperone protein DnaK (dnaK), partial CSB-YP803465FGB
CSB-EP803465FGB
CSB-BP803465FGB
CSB-MP803465FGB
CSB-EP803465FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP803466FGB
CSB-EP803466FGB
CSB-BP803466FGB
CSB-MP803466FGB
CSB-EP803466FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP803467FGB
CSB-EP803467FGB
CSB-BP803467FGB
CSB-MP803467FGB
CSB-EP803467FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Sulfoxide reductase catalytic subunit YedY (yedY) CSB-YP803468FGB
CSB-EP803468FGB
CSB-BP803468FGB
CSB-MP803468FGB
CSB-EP803468FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0745 protein PP_4590 (PP_4590) CSB-YP803469FGB
CSB-EP803469FGB
CSB-BP803469FGB
CSB-MP803469FGB
CSB-EP803469FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida FMN-dependent NADH-azoreductase 2 (azoR2) CSB-YP803470FGB
CSB-EP803470FGB
CSB-BP803470FGB
CSB-MP803470FGB
CSB-EP803470FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Flagellar P-ring protein (flgI) CSB-YP803471FGB
CSB-EP803471FGB
CSB-BP803471FGB
CSB-MP803471FGB
CSB-EP803471FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP803472FGB
CSB-EP803472FGB
CSB-BP803472FGB
CSB-MP803472FGB
CSB-EP803472FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0345 protein PP_4248 (PP_4248) CSB-YP803473FGB
CSB-EP803473FGB
CSB-BP803473FGB
CSB-MP803473FGB
CSB-EP803473FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP803474FGB1
CSB-EP803474FGB1
CSB-BP803474FGB1
CSB-MP803474FGB1
CSB-EP803474FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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