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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Antiholin-like protein LrgB (lrgB), partial CSB-YP802374BQE1
CSB-EP802374BQE1
CSB-BP802374BQE1
CSB-MP802374BQE1
CSB-EP802374BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable tautomerase BA_5626/GBAA_5626/BAS5226 (BA_5626, GBAA_5626, BAS5226) CSB-YP802375BQE
CSB-EP802375BQE
CSB-BP802375BQE
CSB-MP802375BQE
CSB-EP802375BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L31 type B (rpmE2) CSB-YP802376BQE
CSB-EP802376BQE
CSB-BP802376BQE
CSB-MP802376BQE
CSB-EP802376BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-specific phosphotransferase enzyme IIA component (crr) CSB-YP802377BQE
CSB-EP802377BQE
CSB-BP802377BQE
CSB-MP802377BQE
CSB-EP802377BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase gamma chain (atpG) CSB-YP802378BQE
CSB-EP802378BQE
CSB-BP802378BQE
CSB-MP802378BQE
CSB-EP802378BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP802379BQE
CSB-EP802379BQE
CSB-BP802379BQE
CSB-MP802379BQE
CSB-EP802379BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-lactate dehydrogenase 2 (ldh2) CSB-YP802380BQE
CSB-EP802380BQE
CSB-BP802380BQE
CSB-MP802380BQE
CSB-EP802380BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative membrane protein insertion efficiency factor (BA_5048, GBAA_5048, BAS4688) CSB-YP802381BQE
CSB-EP802381BQE
CSB-BP802381BQE
CSB-MP802381BQE
CSB-EP802381BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable molybdenum cofactor guanylyltransferase (mobA) CSB-YP802382BQE
CSB-EP802382BQE
CSB-BP802382BQE
CSB-MP802382BQE
CSB-EP802382BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Large-conductance mechanosensitive channel (mscL), partial CSB-YP802383BQE1
CSB-EP802383BQE1
CSB-BP802383BQE1
CSB-MP802383BQE1
CSB-EP802383BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable inorganic polyphosphate/ATP-NAD kinase 2 (ppnK2) CSB-YP802384BQE
CSB-EP802384BQE
CSB-BP802384BQE
CSB-MP802384BQE
CSB-EP802384BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP802385BQE
CSB-EP802385BQE
CSB-BP802385BQE
CSB-MP802385BQE
CSB-EP802385BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-3 (infC) CSB-YP802386BQE
CSB-EP802386BQE
CSB-BP802386BQE
CSB-MP802386BQE
CSB-EP802386BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant MutS2 protein (mutS2), partial CSB-YP802387BQE
CSB-EP802387BQE
CSB-BP802387BQE
CSB-MP802387BQE
CSB-EP802387BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP802388BQE
CSB-EP802388BQE
CSB-BP802388BQE
CSB-MP802388BQE
CSB-EP802388BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L21 (rplU) CSB-YP802389BQE
CSB-EP802389BQE
CSB-BP802389BQE
CSB-MP802389BQE
CSB-EP802389BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP802390BQE
CSB-EP802390BQE
CSB-BP802390BQE
CSB-MP802390BQE
CSB-EP802390BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alanine--tRNA ligase (alaS), partial CSB-YP802391BQE
CSB-EP802391BQE
CSB-BP802391BQE
CSB-MP802391BQE
CSB-EP802391BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcription elongation factor GreA (greA) CSB-YP802392BQE
CSB-EP802392BQE
CSB-BP802392BQE
CSB-MP802392BQE
CSB-EP802392BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaK (dnaK), partial CSB-YP802393BQE
CSB-EP802393BQE
CSB-BP802393BQE
CSB-MP802393BQE
CSB-EP802393BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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