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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP802394BQE
CSB-EP802394BQE
CSB-BP802394BQE
CSB-MP802394BQE
CSB-EP802394BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-dehydroquinate dehydratase (aroQ) CSB-YP802395BQE
CSB-EP802395BQE
CSB-BP802395BQE
CSB-MP802395BQE
CSB-EP802395BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP802396BQE
CSB-EP802396BQE
CSB-BP802396BQE
CSB-MP802396BQE
CSB-EP802396BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetylglutamate kinase (argB) CSB-YP802397BQE
CSB-EP802397BQE
CSB-BP802397BQE
CSB-MP802397BQE
CSB-EP802397BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anti-sigma F factor (spoIIAB) CSB-YP802398BQE
CSB-EP802398BQE
CSB-BP802398BQE
CSB-MP802398BQE
CSB-EP802398BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW) CSB-YP802399BQE
CSB-EP802399BQE
CSB-BP802399BQE
CSB-MP802399BQE
CSB-EP802399BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0356 protein BA_4189/GBAA_4189/BAS3886 (BA_4189, GBAA_4189, BAS3886) CSB-YP802400BQE
CSB-EP802400BQE
CSB-BP802400BQE
CSB-MP802400BQE
CSB-EP802400BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protoheme IX farnesyltransferase (ctaB), partial CSB-YP802401BQE
CSB-EP802401BQE
CSB-BP802401BQE
CSB-MP802401BQE
CSB-EP802401BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative ABC transporter ATP-binding protein BA_3364/GBAA_3364/BAS3118 (BA_3364, GBAA_3364, BAS3118), partial CSB-YP802402BQE
CSB-EP802402BQE
CSB-BP802402BQE
CSB-MP802402BQE
CSB-EP802402BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrrolidone-carboxylate peptidase (pcp) CSB-YP802403BQE
CSB-EP802403BQE
CSB-BP802403BQE
CSB-MP802403BQE
CSB-EP802403BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chorismate synthase 2 (aroC2) CSB-YP802404BQE
CSB-EP802404BQE
CSB-BP802404BQE
CSB-MP802404BQE
CSB-EP802404BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein BA_2834/GBAA_2834/BAS2643 (BA_2834, GBAA_2834, BAS2643) CSB-YP802405BQE
CSB-EP802405BQE
CSB-BP802405BQE
CSB-MP802405BQE
CSB-EP802405BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0457 protein BA_2525/GBAA_2525/BAS2348 (BA_2525, GBAA_2525, BAS2348) CSB-YP802406BQE
CSB-EP802406BQE
CSB-BP802406BQE
CSB-MP802406BQE
CSB-EP802406BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol-3-phosphate acyltransferase 1 (plsY1), partial CSB-YP802407BQE1
CSB-EP802407BQE1
CSB-BP802407BQE1
CSB-MP802407BQE1
CSB-EP802407BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosome-anchoring protein racA (racA) CSB-YP802408BQE
CSB-EP802408BQE
CSB-BP802408BQE
CSB-MP802408BQE
CSB-EP802408BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Formate--tetrahydrofolate ligase (fhs), partial CSB-YP802409BQE
CSB-EP802409BQE
CSB-BP802409BQE
CSB-MP802409BQE
CSB-EP802409BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0291 protein BA_1897/GBAA_1897/BAS1759 (BA_1897, GBAA_1897, BAS1759) CSB-YP802410BQE
CSB-EP802410BQE
CSB-BP802410BQE
CSB-MP802410BQE
CSB-EP802410BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ketol-acid reductoisomerase 2 (ilvC2) CSB-YP802411BQE
CSB-EP802411BQE
CSB-BP802411BQE
CSB-MP802411BQE
CSB-EP802411BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction resolvase recU (recU) CSB-YP802412BQE
CSB-EP802412BQE
CSB-BP802412BQE
CSB-MP802412BQE
CSB-EP802412BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0302 protein BA_1542/GBAA_1542/BAS1430 (BA_1542, GBAA_1542, BAS1430) CSB-YP802413BQE
CSB-EP802413BQE
CSB-BP802413BQE
CSB-MP802413BQE
CSB-EP802413BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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