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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Nitrosomonas europaea Peptide deformylase 2 (def2) CSB-YP802594NHH
CSB-EP802594NHH
CSB-BP802594NHH
CSB-MP802594NHH
CSB-EP802594NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea K (+)-insensitive pyrophosphate-energized proton pump (hppA), partial CSB-YP802595NHH
CSB-EP802595NHH
CSB-BP802595NHH
CSB-MP802595NHH
CSB-EP802595NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP802596NHH
CSB-EP802596NHH
CSB-BP802596NHH
CSB-MP802596NHH
CSB-EP802596NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP802597NHH
CSB-EP802597NHH
CSB-BP802597NHH
CSB-MP802597NHH
CSB-EP802597NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Ribose-phosphate pyrophosphokinase (prs) CSB-YP802598NHH
CSB-EP802598NHH
CSB-BP802598NHH
CSB-MP802598NHH
CSB-EP802598NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP802599NHH
CSB-EP802599NHH
CSB-BP802599NHH
CSB-MP802599NHH
CSB-EP802599NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Peptide deformylase 1 (def1) CSB-YP802600NHH
CSB-EP802600NHH
CSB-BP802600NHH
CSB-MP802600NHH
CSB-EP802600NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP802601NHH
CSB-EP802601NHH
CSB-BP802601NHH
CSB-MP802601NHH
CSB-EP802601NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea UPF0301 protein NE1668 (NE1668) CSB-YP802602NHH
CSB-EP802602NHH
CSB-BP802602NHH
CSB-MP802602NHH
CSB-EP802602NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Maf-like protein NE1642 (NE1642) CSB-YP802603NHH
CSB-EP802603NHH
CSB-BP802603NHH
CSB-MP802603NHH
CSB-EP802603NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP802604NHH
CSB-EP802604NHH
CSB-BP802604NHH
CSB-MP802604NHH
CSB-EP802604NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP802605NHH
CSB-EP802605NHH
CSB-BP802605NHH
CSB-MP802605NHH
CSB-EP802605NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP802606NHH
CSB-EP802606NHH
CSB-BP802606NHH
CSB-MP802606NHH
CSB-EP802606NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Alanine racemase (alr) CSB-YP802607NHH
CSB-EP802607NHH
CSB-BP802607NHH
CSB-MP802607NHH
CSB-EP802607NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea 50S ribosomal protein L21 (rplU) CSB-YP802608NHH
CSB-EP802608NHH
CSB-BP802608NHH
CSB-MP802608NHH
CSB-EP802608NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP802609NHH
CSB-EP802609NHH
CSB-BP802609NHH
CSB-MP802609NHH
CSB-EP802609NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Macrolide export ATP-binding/permease protein MacB (macB), partial CSB-YP802610NHH1
CSB-EP802610NHH1
CSB-BP802610NHH1
CSB-MP802610NHH1
CSB-EP802610NHH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea D-alanine--D-alanine ligase (ddl) CSB-YP802611NHH
CSB-EP802611NHH
CSB-BP802611NHH
CSB-MP802611NHH
CSB-EP802611NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Protein MraZ (mraZ) CSB-YP802612NHH
CSB-EP802612NHH
CSB-BP802612NHH
CSB-MP802612NHH
CSB-EP802612NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosomonas europaea Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP802613NHH
CSB-EP802613NHH
CSB-BP802613NHH
CSB-MP802613NHH
CSB-EP802613NHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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