Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Spiroplasma virus SpV1-C74 Uncharacterized protein ORF15 (ORF15), partial CSB-YP803395SGAH1
CSB-EP803395SGAH1
CSB-BP803395SGAH1
CSB-MP803395SGAH1
CSB-EP803395SGAH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus SpV1-C74 Uncharacterized protein ORF7 (ORF7), partial CSB-YP803396SGAH1
CSB-EP803396SGAH1
CSB-BP803396SGAH1
CSB-MP803396SGAH1
CSB-EP803396SGAH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian virus 5 RNA-directed RNA polymerase L (L), partial CSB-YP803397SHU
CSB-EP803397SHU
CSB-BP803397SHU
CSB-MP803397SHU
CSB-EP803397SHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato tRNA-dihydrouridine synthase A (dusA) CSB-YP803398FGP
CSB-EP803398FGP
CSB-BP803398FGP
CSB-MP803398FGP
CSB-EP803398FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Acireductone dioxygenase (mtnD) CSB-YP803399FGP
CSB-EP803399FGP
CSB-BP803399FGP
CSB-MP803399FGP
CSB-EP803399FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UPF0345 protein PSPTO_2022 (PSPTO_2022) CSB-YP803400FGP
CSB-EP803400FGP
CSB-BP803400FGP
CSB-MP803400FGP
CSB-EP803400FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Flagellar hook-basal body complex protein FliE (fliE) CSB-YP803401FGP
CSB-EP803401FGP
CSB-BP803401FGP
CSB-MP803401FGP
CSB-EP803401FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Succinylglutamate desuccinylase (astE) CSB-YP803402FGP
CSB-EP803402FGP
CSB-BP803402FGP
CSB-MP803402FGP
CSB-EP803402FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Pterin-4-alpha-carbinolamine dehydratase (phhB) CSB-YP803403FGP
CSB-EP803403FGP
CSB-BP803403FGP
CSB-MP803403FGP
CSB-EP803403FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Cytidylate kinase (cmk) CSB-YP803404FGP
CSB-EP803404FGP
CSB-BP803404FGP
CSB-MP803404FGP
CSB-EP803404FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP803405FGP
CSB-EP803405FGP
CSB-BP803405FGP
CSB-MP803405FGP
CSB-EP803405FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP803406FGP
CSB-EP803406FGP
CSB-BP803406FGP
CSB-MP803406FGP
CSB-EP803406FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UPF0337 protein PSPTO_1596 (PSPTO_1596) CSB-YP803407FGP
CSB-EP803407FGP
CSB-BP803407FGP
CSB-MP803407FGP
CSB-EP803407FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Enolase 1 (eno1) CSB-YP803408FGP
CSB-EP803408FGP
CSB-BP803408FGP
CSB-MP803408FGP
CSB-EP803408FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP803409FGP
CSB-EP803409FGP
CSB-BP803409FGP
CSB-MP803409FGP
CSB-EP803409FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Elongation factor Ts (tsf) CSB-YP803410FGP
CSB-EP803410FGP
CSB-BP803410FGP
CSB-MP803410FGP
CSB-EP803410FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Chemotaxis response regulator protein-glutamate methylesterase of group 3 operon (cheB3) CSB-YP803411FGP
CSB-EP803411FGP
CSB-BP803411FGP
CSB-MP803411FGP
CSB-EP803411FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP803412FGP
CSB-EP803412FGP
CSB-BP803412FGP
CSB-MP803412FGP
CSB-EP803412FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP803413FGP
CSB-EP803413FGP
CSB-BP803413FGP
CSB-MP803413FGP
CSB-EP803413FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Effector protein hopM1 (hopM1), partial CSB-YP803414FGP
CSB-EP803414FGP
CSB-BP803414FGP
CSB-MP803414FGP
CSB-EP803414FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>