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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Protein TolB (tolB) CSB-YP802895MXA
CSB-EP802895MXA
CSB-BP802895MXA
CSB-MP802895MXA
CSB-EP802895MXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O78:H11 Serine protease eatA (eatA), partial CSB-YP802896EOM
CSB-EP802896EOM
CSB-BP802896EOM
CSB-MP802896EOM
CSB-EP802896EOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 DNA translocase FtsK (ftsK), partial CSB-YP802897VEX
CSB-EP802897VEX
CSB-BP802897VEX
CSB-MP802897VEX
CSB-EP802897VEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L1 (rplA) CSB-YP802898SYG
CSB-EP802898SYG
CSB-BP802898SYG
CSB-MP802898SYG
CSB-EP802898SYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At2g17670 (At2g17670) CSB-YP802899DOA
CSB-EP802899DOA
CSB-BP802899DOA
CSB-MP802899DOA
CSB-EP802899DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ribosomal RNA-processing protein 8 (At5g40530) CSB-YP802900DOA
CSB-EP802900DOA
CSB-BP802900DOA
CSB-MP802900DOA
CSB-EP802900DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At1g20360 (At1g20360) CSB-YP802901DOA
CSB-EP802901DOA
CSB-BP802901DOA
CSB-MP802901DOA
CSB-EP802901DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peptide methionine sulfoxide reductase B9 (MSRB9) CSB-YP802902DOA
CSB-EP802902DOA
CSB-BP802902DOA
CSB-MP802902DOA
CSB-EP802902DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peroxisomal membrane protein 11E (PEX11E), partial CSB-YP802903DOA1
CSB-EP802903DOA1
CSB-BP802903DOA1
CSB-MP802903DOA1
CSB-EP802903DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter A family member 2 (ABCA2), partial CSB-YP802904DOA
CSB-EP802904DOA
CSB-BP802904DOA
CSB-MP802904DOA
CSB-EP802904DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Ammonium transporter 2 member 1 (AMT2-1), partial CSB-YP802905OFG
CSB-EP802905OFG
CSB-BP802905OFG
CSB-MP802905OFG
CSB-EP802905OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Nuclear cap-binding protein subunit 2 (CBP20) CSB-YP802906OFG
CSB-EP802906OFG
CSB-BP802906OFG
CSB-MP802906OFG
CSB-EP802906OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial CSB-YP802907SZY
CSB-EP802907SZY
CSB-BP802907SZY
CSB-MP802907SZY
CSB-EP802907SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable inactive purple acid phosphatase 14 (PAP14) CSB-YP802908DOA
CSB-EP802908DOA
CSB-BP802908DOA
CSB-MP802908DOA
CSB-EP802908DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Actin-related protein 4 (ARP4) CSB-YP802909DOA
CSB-EP802909DOA
CSB-BP802909DOA
CSB-MP802909DOA
CSB-EP802909DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At4g32430, mitochondrial (PCMP-E40), partial CSB-YP802910DOA
CSB-EP802910DOA
CSB-BP802910DOA
CSB-MP802910DOA
CSB-EP802910DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized GPI-anchored protein At3g06035 (At3g06035) CSB-YP802911DOA
CSB-EP802911DOA
CSB-BP802911DOA
CSB-MP802911DOA
CSB-EP802911DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein MONOCULM 1 (MOC1) CSB-YP802912OFG
CSB-EP802912OFG
CSB-BP802912OFG
CSB-MP802912OFG
CSB-EP802912OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Flavone O-methyltransferase 1 (OMT1) CSB-YP802913TQN
CSB-EP802913TQN
CSB-BP802913TQN
CSB-MP802913TQN
CSB-EP802913TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica MADS-box transcription factor 25 (MADS25) CSB-YP802914OFG
CSB-EP802914OFG
CSB-BP802914OFG
CSB-MP802914OFG
CSB-EP802914OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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