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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP803295VFE
CSB-EP803295VFE
CSB-BP803295VFE
CSB-MP803295VFE
CSB-EP803295VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP803296VFE
CSB-EP803296VFE
CSB-BP803296VFE
CSB-MP803296VFE
CSB-EP803296VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP803297VFE
CSB-EP803297VFE
CSB-BP803297VFE
CSB-MP803297VFE
CSB-EP803297VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0133 protein VP2178 (VP2178) CSB-YP803298VFE
CSB-EP803298VFE
CSB-BP803298VFE
CSB-MP803298VFE
CSB-EP803298VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0352 protein VP2129 (VP2129) CSB-YP803299VFE
CSB-EP803299VFE
CSB-BP803299VFE
CSB-MP803299VFE
CSB-EP803299VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Electron transport complex protein RnfA (rnfA), partial CSB-YP803300VFE1
CSB-EP803300VFE1
CSB-BP803300VFE1
CSB-MP803300VFE1
CSB-EP803300VFE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0208 membrane protein VP2081 (VP2081), partial CSB-YP803301VFE1
CSB-EP803301VFE1
CSB-BP803301VFE1
CSB-MP803301VFE1
CSB-EP803301VFE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L32 (rpmF) CSB-YP803302VFE
CSB-EP803302VFE
CSB-BP803302VFE
CSB-MP803302VFE
CSB-EP803302VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Integration host factor subunit beta (ihfB) CSB-YP803303VFE
CSB-EP803303VFE
CSB-BP803303VFE
CSB-MP803303VFE
CSB-EP803303VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP803304VFE
CSB-EP803304VFE
CSB-BP803304VFE
CSB-MP803304VFE
CSB-EP803304VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative agmatine deiminase (aguA) CSB-YP803305VFE
CSB-EP803305VFE
CSB-BP803305VFE
CSB-MP803305VFE
CSB-EP803305VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 7-cyano-7-deazaguanine synthase (queC) CSB-YP803306VFE
CSB-EP803306VFE
CSB-BP803306VFE
CSB-MP803306VFE
CSB-EP803306VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molybdenum cofactor guanylyltransferase (mobA) CSB-YP803307VFE
CSB-EP803307VFE
CSB-BP803307VFE
CSB-MP803307VFE
CSB-EP803307VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Succinylglutamate desuccinylase (astE) CSB-YP803308VFE
CSB-EP803308VFE
CSB-BP803308VFE
CSB-MP803308VFE
CSB-EP803308VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazolonepropionase (hutI) CSB-YP803309VFE
CSB-EP803309VFE
CSB-BP803309VFE
CSB-MP803309VFE
CSB-EP803309VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoserine aminotransferase (serC) CSB-YP803310VFE
CSB-EP803310VFE
CSB-BP803310VFE
CSB-MP803310VFE
CSB-EP803310VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidine kinase (tdk) CSB-YP803311VFE
CSB-EP803311VFE
CSB-BP803311VFE
CSB-MP803311VFE
CSB-EP803311VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP803312VFE
CSB-EP803312VFE
CSB-BP803312VFE
CSB-MP803312VFE
CSB-EP803312VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 8-amino-7-oxononanoate synthase (bioF) CSB-YP803313VFE
CSB-EP803313VFE
CSB-BP803313VFE
CSB-MP803313VFE
CSB-EP803313VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Quinolinate synthase A (nadA) CSB-YP803314VFE
CSB-EP803314VFE
CSB-BP803314VFE
CSB-MP803314VFE
CSB-EP803314VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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