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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas putida Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP803495FGB
CSB-EP803495FGB
CSB-BP803495FGB
CSB-MP803495FGB
CSB-EP803495FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Elongation factor Ts (tsf) CSB-YP803496FGB
CSB-EP803496FGB
CSB-BP803496FGB
CSB-MP803496FGB
CSB-EP803496FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP803497FGB
CSB-EP803497FGB
CSB-BP803497FGB
CSB-MP803497FGB
CSB-EP803497FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L19 (rplS) CSB-YP803498FGB
CSB-EP803498FGB
CSB-BP803498FGB
CSB-MP803498FGB
CSB-EP803498FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida tRNA (mo5U34)-methyltransferase (cmoB) CSB-YP803499FGB
CSB-EP803499FGB
CSB-BP803499FGB
CSB-MP803499FGB
CSB-EP803499FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Elongation factor 4 (lepA), partial CSB-YP803500FGB
CSB-EP803500FGB
CSB-BP803500FGB
CSB-MP803500FGB
CSB-EP803500FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable efflux pump outer membrane protein ttgC (ttgC) CSB-YP803501FGB
CSB-EP803501FGB
CSB-BP803501FGB
CSB-MP803501FGB
CSB-EP803501FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida D-alanine--D-alanine ligase B (ddlB) CSB-YP803502FGB
CSB-EP803502FGB
CSB-BP803502FGB
CSB-MP803502FGB
CSB-EP803502FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Protein MraZ (mraZ) CSB-YP803503FGB
CSB-EP803503FGB
CSB-BP803503FGB
CSB-MP803503FGB
CSB-EP803503FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida ATP-dependent RNA helicase rhlB (rhlB) CSB-YP803504FGB
CSB-EP803504FGB
CSB-BP803504FGB
CSB-MP803504FGB
CSB-EP803504FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable poly (beta-D-mannuronate) O-acetylase (algI), partial CSB-YP803505FGB
CSB-EP803505FGB
CSB-BP803505FGB
CSB-MP803505FGB
CSB-EP803505FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP803506FGB
CSB-EP803506FGB
CSB-BP803506FGB
CSB-MP803506FGB
CSB-EP803506FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida C4-dicarboxylate transport protein (dctA), partial CSB-YP803507FGB
CSB-EP803507FGB
CSB-BP803507FGB
CSB-MP803507FGB
CSB-EP803507FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Acyl-homoserine lactone acylase quiP (quiP), partial CSB-YP803508FGB
CSB-EP803508FGB
CSB-BP803508FGB
CSB-MP803508FGB
CSB-EP803508FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0276 protein PP_0992 (PP_0992) CSB-YP803509FGB
CSB-EP803509FGB
CSB-BP803509FGB
CSB-MP803509FGB
CSB-EP803509FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Nucleoid-associated protein PP_0973 (PP_0973) CSB-YP803510FGB
CSB-EP803510FGB
CSB-BP803510FGB
CSB-MP803510FGB
CSB-EP803510FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Maf-like protein PP_0936 (maf-1) CSB-YP803511FGB
CSB-EP803511FGB
CSB-BP803511FGB
CSB-MP803511FGB
CSB-EP803511FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Peptide chain release factor 3 (prfC), partial CSB-YP803512FGB
CSB-EP803512FGB
CSB-BP803512FGB
CSB-MP803512FGB
CSB-EP803512FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Chaperone protein hscA homolog (hscA), partial CSB-YP803513FGB
CSB-EP803513FGB
CSB-BP803513FGB
CSB-MP803513FGB
CSB-EP803513FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida tRNA 2-selenouridine synthase (selU) CSB-YP803514FGB
CSB-EP803514FGB
CSB-BP803514FGB
CSB-MP803514FGB
CSB-EP803514FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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