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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana 4-coumarate--CoA ligase-like 6 (4CLL6), partial CSB-YP802915DOA
CSB-EP802915DOA
CSB-BP802915DOA
CSB-MP802915DOA
CSB-EP802915DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Zinc finger A20 and AN1 domain-containing stress-associated protein 11 (SAP11) CSB-YP802916OFG
CSB-EP802916OFG
CSB-BP802916OFG
CSB-MP802916OFG
CSB-EP802916OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica ASC1-like protein 3 (Os03g0264000, LOC_Os03g15750), partial CSB-YP802917OFG1
CSB-EP802917OFG1
CSB-BP802917OFG1
CSB-MP802917OFG1
CSB-EP802917OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DUF21 domain-containing protein At1g55930, chloroplastic (CBSDUFCH2), partial CSB-YP802918DOA1
CSB-EP802918DOA1
CSB-BP802918DOA1
CSB-MP802918DOA1
CSB-EP802918DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NifU-like protein 3, chloroplastic (NIFU3) CSB-YP802919DOA
CSB-EP802919DOA
CSB-BP802919DOA
CSB-MP802919DOA
CSB-EP802919DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable mixed-linked glucan synthase 8 (CSFL8), partial CSB-YP802920OFG
CSB-EP802920OFG
CSB-BP802920OFG
CSB-MP802920OFG
CSB-EP802920OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Exosome complex exonuclease RRP46 homolog (RRP46) CSB-YP802921OFG
CSB-EP802921OFG
CSB-BP802921OFG
CSB-MP802921OFG
CSB-EP802921OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NAC domain-containing protein 21/22 (NAC021) CSB-YP802922DOA
CSB-EP802922DOA
CSB-BP802922DOA
CSB-MP802922DOA
CSB-EP802922DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin beta chain (TUBB1) CSB-YP802923EXX
CSB-EP802923EXX
CSB-BP802923EXX
CSB-MP802923EXX
CSB-EP802923EXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Sodium transporter HKT1 (HKT1), partial CSB-YP802924DOA
CSB-EP802924DOA
CSB-BP802924DOA
CSB-MP802924DOA
CSB-EP802924DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica SCAR-like protein 1 (Os03g0816900, Os03g0817000, LOC_Os03g60240), partial CSB-YP802925OFG
CSB-EP802925OFG
CSB-BP802925OFG
CSB-MP802925OFG
CSB-EP802925OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica DEAD-box ATP-dependent RNA helicase 42 (Os08g0159900, LOC_Os08g06344), partial CSB-YP802926OFG
CSB-EP802926OFG
CSB-BP802926OFG
CSB-MP802926OFG
CSB-EP802926OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Terpenoid synthase 6 (TPS06), partial CSB-YP802927DOA
CSB-EP802927DOA
CSB-BP802927DOA
CSB-MP802927DOA
CSB-EP802927DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein BRICK1 (Os02g0829900, LOC_Os02g58320) CSB-YP802928OFG
CSB-EP802928OFG
CSB-BP802928OFG
CSB-MP802928OFG
CSB-EP802928OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana SNF1-related protein kinase regulatory subunit beta-1 (KINB1) CSB-YP802929DOA
CSB-EP802929DOA
CSB-BP802929DOA
CSB-MP802929DOA
CSB-EP802929DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable SAL4 phosphatase (SAL4) CSB-YP802930DOA
CSB-EP802930DOA
CSB-BP802930DOA
CSB-MP802930DOA
CSB-EP802930DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutamate receptor 3.6 (GLR3.6), partial CSB-YP802931DOA
CSB-EP802931DOA
CSB-BP802931DOA
CSB-MP802931DOA
CSB-EP802931DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable disease resistance protein At4g19060 (At4g19060) CSB-YP802932DOA
CSB-EP802932DOA
CSB-BP802932DOA
CSB-MP802932DOA
CSB-EP802932DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable DNA primase large subunit (At1g67320) CSB-YP802933DOA
CSB-EP802933DOA
CSB-BP802933DOA
CSB-MP802933DOA
CSB-EP802933DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At4g37920, chloroplastic (At4g37920) CSB-YP802934DOA
CSB-EP802934DOA
CSB-BP802934DOA
CSB-MP802934DOA
CSB-EP802934DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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