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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bradyrhizobium japonicum Large-conductance mechanosensitive channel (mscL), partial CSB-YP803730BVW1
CSB-EP803730BVW1
CSB-BP803730BVW1
CSB-MP803730BVW1
CSB-EP803730BVW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Trigger factor (tig) CSB-YP803731BVW
CSB-EP803731BVW
CSB-BP803731BVW
CSB-MP803731BVW
CSB-EP803731BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Proline--tRNA ligase (proS) CSB-YP803732BVW
CSB-EP803732BVW
CSB-BP803732BVW
CSB-MP803732BVW
CSB-EP803732BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP803733BVW
CSB-EP803733BVW
CSB-BP803733BVW
CSB-MP803733BVW
CSB-EP803733BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glutamate--tRNA ligase (gltX) CSB-YP803734BVW
CSB-EP803734BVW
CSB-BP803734BVW
CSB-MP803734BVW
CSB-EP803734BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Enolase (eno) CSB-YP803735BVW
CSB-EP803735BVW
CSB-BP803735BVW
CSB-MP803735BVW
CSB-EP803735BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Single-stranded DNA-binding protein (ssb) CSB-YP803736BVW
CSB-EP803736BVW
CSB-BP803736BVW
CSB-MP803736BVW
CSB-EP803736BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UPF0271 protein blr4568 (blr4568) CSB-YP803737BVW
CSB-EP803737BVW
CSB-BP803737BVW
CSB-MP803737BVW
CSB-EP803737BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Bifunctional enzyme IspD/IspF (ispDF) CSB-YP803738BVW
CSB-EP803738BVW
CSB-BP803738BVW
CSB-MP803738BVW
CSB-EP803738BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Cell division topological specificity factor (minE) CSB-YP803739BVW
CSB-EP803739BVW
CSB-BP803739BVW
CSB-MP803739BVW
CSB-EP803739BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 4-hydroxythreonine-4-phosphate dehydrogenase 2 (pdxA2) CSB-YP803740BVW
CSB-EP803740BVW
CSB-BP803740BVW
CSB-MP803740BVW
CSB-EP803740BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UPF0178 protein bll3966 (bll3966) CSB-YP803741BVW
CSB-EP803741BVW
CSB-BP803741BVW
CSB-MP803741BVW
CSB-EP803741BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP803742BVW
CSB-EP803742BVW
CSB-BP803742BVW
CSB-MP803742BVW
CSB-EP803742BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Uncharacterized transporter bll3475 (bll3475), partial CSB-YP803743BVW
CSB-EP803743BVW
CSB-BP803743BVW
CSB-MP803743BVW
CSB-EP803743BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Cobalamin synthase (cobS), partial CSB-YP803744BVW1
CSB-EP803744BVW1
CSB-BP803744BVW1
CSB-MP803744BVW1
CSB-EP803744BVW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UPF0260 protein blr3103 (blr3103) CSB-YP803745BVW
CSB-EP803745BVW
CSB-BP803745BVW
CSB-MP803745BVW
CSB-EP803745BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glycogen synthase 1 (glgA1) CSB-YP803746BVW
CSB-EP803746BVW
CSB-BP803746BVW
CSB-MP803746BVW
CSB-EP803746BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP803747BVW
CSB-EP803747BVW
CSB-BP803747BVW
CSB-MP803747BVW
CSB-EP803747BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Probable phosphoketolase (bll2518), partial CSB-YP803748BVW
CSB-EP803748BVW
CSB-BP803748BVW
CSB-MP803748BVW
CSB-EP803748BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon (cheB2) CSB-YP803749BVW
CSB-EP803749BVW
CSB-BP803749BVW
CSB-MP803749BVW
CSB-EP803749BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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