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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium japonicum Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP803750BVW
CSB-EP803750BVW
CSB-BP803750BVW
CSB-MP803750BVW
CSB-EP803750BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UPF0337 protein bsl1473 (bsl1473) CSB-YP803751BVW
CSB-EP803751BVW
CSB-BP803751BVW
CSB-MP803751BVW
CSB-EP803751BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Translation initiation factor IF-1 (infA) CSB-YP803752BVW
CSB-EP803752BVW
CSB-BP803752BVW
CSB-MP803752BVW
CSB-EP803752BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Ribonuclease H (rnhA) CSB-YP803753BVW
CSB-EP803753BVW
CSB-BP803753BVW
CSB-MP803753BVW
CSB-EP803753BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Protein ApaG (apaG) CSB-YP803754BVW
CSB-EP803754BVW
CSB-BP803754BVW
CSB-MP803754BVW
CSB-EP803754BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Aminoacyl carrier protein 1 (bsr0959) CSB-YP803755BVW
CSB-EP803755BVW
CSB-BP803755BVW
CSB-MP803755BVW
CSB-EP803755BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum DNA mismatch repair protein MutS (mutS), partial CSB-YP803756BVW
CSB-EP803756BVW
CSB-BP803756BVW
CSB-MP803756BVW
CSB-EP803756BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP803757BVW
CSB-EP803757BVW
CSB-BP803757BVW
CSB-MP803757BVW
CSB-EP803757BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP803758BVW
CSB-EP803758BVW
CSB-BP803758BVW
CSB-MP803758BVW
CSB-EP803758BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Tryptophan synthase beta chain (trpB) CSB-YP803759BVW
CSB-EP803759BVW
CSB-BP803759BVW
CSB-MP803759BVW
CSB-EP803759BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Undecaprenyl-diphosphatase (uppP), partial CSB-YP803760BVW
CSB-EP803760BVW
CSB-BP803760BVW
CSB-MP803760BVW
CSB-EP803760BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Non-canonical purine NTP pyrophosphatase (bll0673) CSB-YP803761BVW
CSB-EP803761BVW
CSB-BP803761BVW
CSB-MP803761BVW
CSB-EP803761BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UPF0314 protein bll0647 (bll0647), partial CSB-YP803762BVW1
CSB-EP803762BVW1
CSB-BP803762BVW1
CSB-MP803762BVW1
CSB-EP803762BVW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP803763BVW
CSB-EP803763BVW
CSB-BP803763BVW
CSB-MP803763BVW
CSB-EP803763BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Inorganic pyrophosphatase (ppa) CSB-YP803764BVW
CSB-EP803764BVW
CSB-BP803764BVW
CSB-MP803764BVW
CSB-EP803764BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Ribosome maturation factor RimM (rimM) CSB-YP803765BVW
CSB-EP803765BVW
CSB-BP803765BVW
CSB-MP803765BVW
CSB-EP803765BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Gamma-glutamyl phosphate reductase (proA) CSB-YP803766BVW
CSB-EP803766BVW
CSB-BP803766BVW
CSB-MP803766BVW
CSB-EP803766BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Homogentisate 1,2-dioxygenase (hmgA) CSB-YP803767BVW
CSB-EP803767BVW
CSB-BP803767BVW
CSB-MP803767BVW
CSB-EP803767BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP803768BDU
CSB-EP803768BDU
CSB-BP803768BDU
CSB-MP803768BDU
CSB-EP803768BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L25 (rplY) CSB-YP803769BDU
CSB-EP803769BDU
CSB-BP803769BDU
CSB-MP803769BDU
CSB-EP803769BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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