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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium japonicum Transcriptional repressor NrdR (nrdR) CSB-YP805121BVW
CSB-EP805121BVW
CSB-BP805121BVW
CSB-MP805121BVW
CSB-EP805121BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 30S ribosomal protein S9 (rpsI) CSB-YP805122BVW
CSB-EP805122BVW
CSB-BP805122BVW
CSB-MP805122BVW
CSB-EP805122BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP805123BVW
CSB-EP805123BVW
CSB-BP805123BVW
CSB-MP805123BVW
CSB-EP805123BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Undecaprenyl pyrophosphate synthase 2 (uppS2) CSB-YP805124BVW
CSB-EP805124BVW
CSB-BP805124BVW
CSB-MP805124BVW
CSB-EP805124BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Lipid-A-disaccharide synthase (lpxB) CSB-YP805125BVW
CSB-EP805125BVW
CSB-BP805125BVW
CSB-MP805125BVW
CSB-EP805125BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP805126BVW
CSB-EP805126BVW
CSB-BP805126BVW
CSB-MP805126BVW
CSB-EP805126BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Serine--tRNA ligase (serS) CSB-YP805127BVW
CSB-EP805127BVW
CSB-BP805127BVW
CSB-MP805127BVW
CSB-EP805127BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Probable potassium transport system protein kup 3 (kup3), partial CSB-YP805128BVW
CSB-EP805128BVW
CSB-BP805128BVW
CSB-MP805128BVW
CSB-EP805128BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Thymidylate kinase (tmk) CSB-YP805129BVW
CSB-EP805129BVW
CSB-BP805129BVW
CSB-MP805129BVW
CSB-EP805129BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Valine--tRNA ligase (valS), partial CSB-YP805130BVW
CSB-EP805130BVW
CSB-BP805130BVW
CSB-MP805130BVW
CSB-EP805130BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 3-dehydroquinate dehydratase 1 (aroQ1) CSB-YP805131BVW
CSB-EP805131BVW
CSB-BP805131BVW
CSB-MP805131BVW
CSB-EP805131BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Nucleoside diphosphate kinase (ndk) CSB-YP805132BVW
CSB-EP805132BVW
CSB-BP805132BVW
CSB-MP805132BVW
CSB-EP805132BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L9 (rplI) CSB-YP805133BVW
CSB-EP805133BVW
CSB-BP805133BVW
CSB-MP805133BVW
CSB-EP805133BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glutaminase 1 (glsA1), partial CSB-YP805134BVW
CSB-EP805134BVW
CSB-BP805134BVW
CSB-MP805134BVW
CSB-EP805134BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Carboxylate-amine ligase bll3764 (bll3764) CSB-YP805135BVW
CSB-EP805135BVW
CSB-BP805135BVW
CSB-MP805135BVW
CSB-EP805135BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Uncharacterized transporter bll3476 (bll3476), partial CSB-YP805136BVW
CSB-EP805136BVW
CSB-BP805136BVW
CSB-MP805136BVW
CSB-EP805136BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Adenine deaminase 1 (ade1), partial CSB-YP805137BVW
CSB-EP805137BVW
CSB-BP805137BVW
CSB-MP805137BVW
CSB-EP805137BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Ethanolamine ammonia-lyase light chain (eutC) CSB-YP805138BVW
CSB-EP805138BVW
CSB-BP805138BVW
CSB-MP805138BVW
CSB-EP805138BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Protein CrcB homolog 1 (crcB1), partial CSB-YP805139BVW1
CSB-EP805139BVW1
CSB-BP805139BVW1
CSB-MP805139BVW1
CSB-EP805139BVW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 3-methyl-2-oxobutanoate hydroxymethyltransferase 1 (panB1) CSB-YP805140BVW
CSB-EP805140BVW
CSB-BP805140BVW
CSB-MP805140BVW
CSB-EP805140BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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