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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacteroides thetaiotaomicron Dihydroxy-acid dehydratase (ilvD), partial CSB-YP804265BDU
CSB-EP804265BDU
CSB-BP804265BDU
CSB-MP804265BDU
CSB-EP804265BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Ribosome maturation factor RimM (rimM) CSB-YP804266BDU
CSB-EP804266BDU
CSB-BP804266BDU
CSB-MP804266BDU
CSB-EP804266BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP804267BDU
CSB-EP804267BDU
CSB-BP804267BDU
CSB-MP804267BDU
CSB-EP804267BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase 2 (gpmA2) CSB-YP804268BDU
CSB-EP804268BDU
CSB-BP804268BDU
CSB-MP804268BDU
CSB-EP804268BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Single-stranded DNA-binding protein (ssb) CSB-YP804269BDU
CSB-EP804269BDU
CSB-BP804269BDU
CSB-MP804269BDU
CSB-EP804269BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP804270BDU
CSB-EP804270BDU
CSB-BP804270BDU
CSB-MP804270BDU
CSB-EP804270BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Uncharacterized transporter BT_1229 (BT_1229), partial CSB-YP804271BDU
CSB-EP804271BDU
CSB-BP804271BDU
CSB-MP804271BDU
CSB-EP804271BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 6-phosphofructokinase 1 (pfkA1) CSB-YP804272BDU
CSB-EP804272BDU
CSB-BP804272BDU
CSB-MP804272BDU
CSB-EP804272BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Chaperone protein htpG (htpG), partial CSB-YP804273BDU
CSB-EP804273BDU
CSB-BP804273BDU
CSB-MP804273BDU
CSB-EP804273BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Uronate isomerase (uxaC) CSB-YP804274BDU
CSB-EP804274BDU
CSB-BP804274BDU
CSB-MP804274BDU
CSB-EP804274BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Serine hydroxymethyltransferase (glyA) CSB-YP804275BDU
CSB-EP804275BDU
CSB-BP804275BDU
CSB-MP804275BDU
CSB-EP804275BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP804276BDU
CSB-EP804276BDU
CSB-BP804276BDU
CSB-MP804276BDU
CSB-EP804276BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Probable transcriptional regulatory protein BT_0627 (BT_0627) CSB-YP804277BDU
CSB-EP804277BDU
CSB-BP804277BDU
CSB-MP804277BDU
CSB-EP804277BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Anthranilate phosphoribosyltransferase (trpD) CSB-YP804278BDU
CSB-EP804278BDU
CSB-BP804278BDU
CSB-MP804278BDU
CSB-EP804278BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Translation initiation factor IF-3 (infC) CSB-YP804279BDU
CSB-EP804279BDU
CSB-BP804279BDU
CSB-MP804279BDU
CSB-EP804279BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron L-arabinose isomerase (araA), partial CSB-YP804280BDU
CSB-EP804280BDU
CSB-BP804280BDU
CSB-MP804280BDU
CSB-EP804280BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron tRNA-specific 2-thiouridylase mnmA 1 (mnmA1) CSB-YP804281BDU
CSB-EP804281BDU
CSB-BP804281BDU
CSB-MP804281BDU
CSB-EP804281BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Protein Vpr CSB-YP804282SIAA
CSB-EP804282SIAA
CSB-BP804282SIAA
CSB-MP804282SIAA
CSB-EP804282SIAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Pericentriolar material 1 protein (PCM1), partial CSB-YP804283CH
CSB-EP804283CH
CSB-BP804283CH
CSB-MP804283CH
CSB-EP804283CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudechis porphyriacus Pseudecin CSB-YP804284EZT
CSB-EP804284EZT
CSB-BP804284EZT
CSB-MP804284EZT
CSB-EP804284EZT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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