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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium japonicum Membrane protein insertase YidC (yidC), partial CSB-YP805101BVW1
CSB-EP805101BVW1
CSB-BP805101BVW1
CSB-MP805101BVW1
CSB-EP805101BVW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Probable acetoacetate decarboxylase 1 (adc1) CSB-YP805102BVW
CSB-EP805102BVW
CSB-BP805102BVW
CSB-MP805102BVW
CSB-EP805102BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Exodeoxyribonuclease 7 large subunit (xseA), partial CSB-YP805103BVW
CSB-EP805103BVW
CSB-BP805103BVW
CSB-MP805103BVW
CSB-EP805103BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Ribose-phosphate pyrophosphokinase (prs) CSB-YP805104BVW
CSB-EP805104BVW
CSB-BP805104BVW
CSB-MP805104BVW
CSB-EP805104BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UPF0182 protein bll7333 (bll7333), partial CSB-YP805105BVW
CSB-EP805105BVW
CSB-BP805105BVW
CSB-MP805105BVW
CSB-EP805105BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Aquaporin Z (aqpZ), partial CSB-YP805106BVW1
CSB-EP805106BVW1
CSB-BP805106BVW1
CSB-MP805106BVW1
CSB-EP805106BVW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Flagellar L-ring protein 2 (flgH2) CSB-YP805107BVW
CSB-EP805107BVW
CSB-BP805107BVW
CSB-MP805107BVW
CSB-EP805107BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Coenzyme PQQ synthesis protein A (pqqA) CSB-YP805108BVW
CSB-EP805108BVW
CSB-BP805108BVW
CSB-MP805108BVW
CSB-EP805108BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP805109BVW
CSB-EP805109BVW
CSB-BP805109BVW
CSB-MP805109BVW
CSB-EP805109BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Cell division protein FtsQ (ftsQ), partial CSB-YP805110BVW
CSB-EP805110BVW
CSB-BP805110BVW
CSB-MP805110BVW
CSB-EP805110BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum D-alanine--D-alanine ligase A (ddlA) CSB-YP805111BVW
CSB-EP805111BVW
CSB-BP805111BVW
CSB-MP805111BVW
CSB-EP805111BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glutathione-dependent formaldehyde-activating enzyme (gfa) CSB-YP805112BVW
CSB-EP805112BVW
CSB-BP805112BVW
CSB-MP805112BVW
CSB-EP805112BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Probable 5-dehydro-4-deoxyglucarate dehydratase (blr5871) CSB-YP805113BVW
CSB-EP805113BVW
CSB-BP805113BVW
CSB-MP805113BVW
CSB-EP805113BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Phosphoribosylaminoimidazole-succinocarboxamide synthase A (purC1) CSB-YP805114BVW
CSB-EP805114BVW
CSB-BP805114BVW
CSB-MP805114BVW
CSB-EP805114BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L1 (rplA) CSB-YP805115BVW
CSB-EP805115BVW
CSB-BP805115BVW
CSB-MP805115BVW
CSB-EP805115BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L3 (rplC) CSB-YP805116BVW
CSB-EP805116BVW
CSB-BP805116BVW
CSB-MP805116BVW
CSB-EP805116BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L24 (rplX) CSB-YP805117BVW
CSB-EP805117BVW
CSB-BP805117BVW
CSB-MP805117BVW
CSB-EP805117BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 30S ribosomal protein S13 (rpsM) CSB-YP805118BVW
CSB-EP805118BVW
CSB-BP805118BVW
CSB-MP805118BVW
CSB-EP805118BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum ATP-dependent Clp protease adapter protein ClpS 2 (clpS2) CSB-YP805119BVW
CSB-EP805119BVW
CSB-BP805119BVW
CSB-MP805119BVW
CSB-EP805119BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP805120BVW
CSB-EP805120BVW
CSB-BP805120BVW
CSB-MP805120BVW
CSB-EP805120BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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