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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacteroides thetaiotaomicron Peptide chain release factor 1 (prfA) CSB-YP804244BDU
CSB-EP804244BDU
CSB-BP804244BDU
CSB-MP804244BDU
CSB-EP804244BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Lipoyl synthase (lipA) CSB-YP804245BDU
CSB-EP804245BDU
CSB-BP804245BDU
CSB-MP804245BDU
CSB-EP804245BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP804246BDU
CSB-EP804246BDU
CSB-BP804246BDU
CSB-MP804246BDU
CSB-EP804246BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Methionyl-tRNA formyltransferase (fmt) CSB-YP804247BDU
CSB-EP804247BDU
CSB-BP804247BDU
CSB-MP804247BDU
CSB-EP804247BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Malate dehydrogenase (mdh) CSB-YP804248BDU
CSB-EP804248BDU
CSB-BP804248BDU
CSB-MP804248BDU
CSB-EP804248BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron UPF0246 protein BT_3869 (BT_3869) CSB-YP804249BDU
CSB-EP804249BDU
CSB-BP804249BDU
CSB-MP804249BDU
CSB-EP804249BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron L-rhamnose isomerase (rhaA) CSB-YP804250BDU
CSB-EP804250BDU
CSB-BP804250BDU
CSB-MP804250BDU
CSB-EP804250BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Putative glycoside hydrolase BT_3595 (BT_3595), partial CSB-YP804252BDU
CSB-EP804252BDU
CSB-BP804252BDU
CSB-MP804252BDU
CSB-EP804252BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron DNA repair protein recO (recO) CSB-YP804253BDU
CSB-EP804253BDU
CSB-BP804253BDU
CSB-MP804253BDU
CSB-EP804253BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Translation initiation factor IF-2 (infB), partial CSB-YP804254BDU
CSB-EP804254BDU
CSB-BP804254BDU
CSB-MP804254BDU
CSB-EP804254BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Probable rRNA maturation factor (BT_3283) CSB-YP804255BDU
CSB-EP804255BDU
CSB-BP804255BDU
CSB-MP804255BDU
CSB-EP804255BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Putative membrane protein insertion efficiency factor (BT_3228) CSB-YP804256BDU
CSB-EP804256BDU
CSB-BP804256BDU
CSB-MP804256BDU
CSB-EP804256BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP804257BDU1
CSB-EP804257BDU1
CSB-BP804257BDU1
CSB-MP804257BDU1
CSB-EP804257BDU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Adenosylhomocysteinase (ahcY) CSB-YP804258BDU
CSB-EP804258BDU
CSB-BP804258BDU
CSB-MP804258BDU
CSB-EP804258BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 30S ribosomal protein S10 (rpsJ) CSB-YP804259BDU
CSB-EP804259BDU
CSB-BP804259BDU
CSB-MP804259BDU
CSB-EP804259BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L16 (rplP) CSB-YP804260BDU
CSB-EP804260BDU
CSB-BP804260BDU
CSB-MP804260BDU
CSB-EP804260BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 30S ribosomal protein S8 (rpsH) CSB-YP804261BDU
CSB-EP804261BDU
CSB-BP804261BDU
CSB-MP804261BDU
CSB-EP804261BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Translation initiation factor IF-1 (infA) CSB-YP804262BDU
CSB-EP804262BDU
CSB-BP804262BDU
CSB-MP804262BDU
CSB-EP804262BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG), partial CSB-YP804263BDU
CSB-EP804263BDU
CSB-BP804263BDU
CSB-MP804263BDU
CSB-EP804263BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Uridylate kinase (pyrH) CSB-YP804264BDU
CSB-EP804264BDU
CSB-BP804264BDU
CSB-MP804264BDU
CSB-EP804264BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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