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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vanderwaltozyma polyspora Ubiquitin carboxyl-terminal hydrolase 4 (DOA4), partial CSB-YP025731VDW
CSB-EP025731VDW
CSB-BP025731VDW
CSB-MP025731VDW
CSB-EP025731VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Conserved oligomeric Golgi complex subunit 6 (COG6), partial CSB-YP005710VDW
CSB-EP005710VDW
CSB-BP005710VDW
CSB-MP005710VDW
CSB-EP005710VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Eukaryotic translation initiation factor 3 subunit B (PRT1), partial CSB-YP007530VDW
CSB-EP007530VDW
CSB-BP007530VDW
CSB-MP007530VDW
CSB-EP007530VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Nucleolar protein 16 (NOP16) CSB-YP015937VDW
CSB-EP015937VDW
CSB-BP015937VDW
CSB-MP015937VDW
CSB-EP015937VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Methylthioribose-1-phosphate isomerase (MRI1) CSB-YP014799VDW
CSB-EP014799VDW
CSB-BP014799VDW
CSB-MP014799VDW
CSB-EP014799VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vanderwaltozyma polyspora Structure-specific endonuclease subunit SLX1 (SLX1) CSB-YP009442VDW
CSB-EP009442VDW
CSB-BP009442VDW
CSB-MP009442VDW
CSB-EP009442VDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oxyrrhis marina Meiosis expressed gene 1 protein homolog CSB-YP013685ODM
CSB-EP013685ODM
CSB-BP013685ODM
CSB-MP013685ODM
CSB-EP013685ODM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Nucleoside diphosphate kinase (ndk) CSB-YP015889SUK
CSB-EP015889SUK
CSB-BP015889SUK
CSB-MP015889SUK
CSB-EP015889SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit b (atpF), partial CSB-YP002358SUK1
CSB-EP002358SUK1
CSB-BP002358SUK1
CSB-MP002358SUK1
CSB-EP002358SUK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide chain release factor 1 (prfA) CSB-YP015197SUK
CSB-EP015197SUK
CSB-BP015197SUK
CSB-MP015197SUK
CSB-EP015197SUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Elongation factor G (fusA), partial CSB-YP009373BQD
CSB-EP009373BQD
CSB-BP009373BQD
CSB-MP009373BQD
CSB-EP009373BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Methionyl-tRNA formyltransferase (fmt) CSB-YP015150BQD
CSB-EP015150BQD
CSB-BP015150BQD
CSB-MP015150BQD
CSB-EP015150BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Acyl carrier protein (acpP) CSB-YP015636BQD
CSB-EP015636BQD
CSB-BP015636BQD
CSB-MP015636BQD
CSB-EP015636BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Elongation factor Ts (tsf) CSB-YP025124BQD
CSB-EP025124BQD
CSB-BP025124BQD
CSB-MP025124BQD
CSB-EP025124BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Malate dehydrogenase (mdh) CSB-YP013623BQD
CSB-EP013623BQD
CSB-BP013623BQD
CSB-MP013623BQD
CSB-EP013623BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Argininosuccinate synthase (argG) CSB-YP002234CWA
CSB-EP002234CWA
CSB-BP002234CWA
CSB-MP002234CWA
CSB-EP002234CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Thymidylate kinase (tmk) CSB-YP007226CWA
CSB-EP007226CWA
CSB-BP007226CWA
CSB-MP007226CWA
CSB-EP007226CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Elongation factor Ts (tsf) CSB-YP025124EJD
CSB-EP025124EJD
CSB-BP025124EJD
CSB-MP025124EJD
CSB-EP025124EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Phosphoserine aminotransferase (serC) CSB-YP018838EJD
CSB-EP018838EJD
CSB-BP018838EJD
CSB-MP018838EJD
CSB-EP018838EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 N-acetyl-D-glucosamine kinase (nagK) CSB-YP015415EJD
CSB-EP015415EJD
CSB-BP015415EJD
CSB-MP015415EJD
CSB-EP015415EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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