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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Parvibaculum lavamentivorans Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917PYE
CSB-EP022917PYE
CSB-BP022917PYE
CSB-MP022917PYE
CSB-EP022917PYE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans tRNA dimethylallyltransferase (miaA) CSB-YP024529PYE
CSB-EP024529PYE
CSB-BP024529PYE
CSB-MP024529PYE
CSB-EP024529PYE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Elongation factor G (fusA), partial CSB-YP009373PYE
CSB-EP009373PYE
CSB-BP009373PYE
CSB-MP009373PYE
CSB-EP009373PYE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parvibaculum lavamentivorans Elongation factor Ts (tsf) CSB-YP025124PYE
CSB-EP025124PYE
CSB-BP025124PYE
CSB-MP025124PYE
CSB-EP025124PYE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis ATP synthase subunit delta (atpH) CSB-YP002355CWD
CSB-EP002355CWD
CSB-BP002355CWD
CSB-MP002355CWD
CSB-EP002355CWD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter hominis ATP synthase subunit a (atpB), partial CSB-YP015070CWD1
CSB-EP015070CWD1
CSB-BP015070CWD1
CSB-MP015070CWD1
CSB-EP015070CWD1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei Nucleoside diphosphate kinase (ndk) CSB-YP015889MNV
CSB-EP015889MNV
CSB-BP015889MNV
CSB-MP015889MNV
CSB-EP015889MNV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Ribosome-recycling factor (frr) CSB-YP014941XAV
CSB-EP014941XAV
CSB-BP014941XAV
CSB-MP014941XAV
CSB-EP014941XAV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pichia angusta Autophagy-related protein 13 (ATG13), partial CSB-YP012203EVQ
CSB-EP012203EVQ
CSB-BP012203EVQ
CSB-MP012203EVQ
CSB-EP012203EVQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium chrysogenum Ubiquitin-like protein ATG12 (atg12) CSB-YP002283ETH
CSB-EP002283ETH
CSB-BP002283ETH
CSB-MP002283ETH
CSB-EP002283ETH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii 3-ketoacyl-CoA thiolase (fadI), partial CSB-YP001117CXL
CSB-EP001117CXL
CSB-BP001117CXL
CSB-MP001117CXL
CSB-EP001117CXL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Elongation factor Tu (tuf1) CSB-YP025342CXL
CSB-EP025342CXL
CSB-BP025342CXL
CSB-MP025342CXL
CSB-EP025342CXL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Argininosuccinate synthase (argG) CSB-YP002234CXL
CSB-EP002234CXL
CSB-BP002234CXL
CSB-MP002234CXL
CSB-EP002234CXL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii ATP synthase subunit a (atpB), partial CSB-YP015070CXL1
CSB-EP015070CXL1
CSB-BP015070CXL1
CSB-MP015070CXL1
CSB-EP015070CXL1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Acetyl-coenzyme A synthetase (acs), partial CSB-YP001203CXL
CSB-EP001203CXL
CSB-BP001203CXL
CSB-MP001203CXL
CSB-EP001203CXL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917CXL
CSB-EP022917CXL
CSB-BP022917CXL
CSB-MP022917CXL
CSB-EP022917CXL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582VEA
CSB-EP009582VEA
CSB-BP009582VEA
CSB-MP009582VEA
CSB-EP009582VEA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Ribosome-recycling factor (frr) CSB-YP014941VEA
CSB-EP014941VEA
CSB-BP014941VEA
CSB-MP014941VEA
CSB-EP014941VEA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi Peptide chain release factor 1 (prfA) CSB-YP015197VEA
CSB-EP015197VEA
CSB-BP015197VEA
CSB-MP015197VEA
CSB-EP015197VEA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. holarctica Ribosome-recycling factor (frr) CSB-YP014941FDV
CSB-EP014941FDV
CSB-BP014941FDV
CSB-MP014941FDV
CSB-EP014941FDV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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