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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Eukaryotic translation initiation factor 3 subunit D (eif-3.D), partial CSB-YP007533CXX
CSB-EP007533CXX
CSB-BP007533CXX
CSB-MP007533CXX
CSB-EP007533CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eukaryotic translation initiation factor 3 subunit L (eif-3.L), partial CSB-YP007541CXX
CSB-EP007541CXX
CSB-BP007541CXX
CSB-MP007541CXX
CSB-EP007541CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calcium/calmodulin-dependent protein kinase type 1 (cmk-1) CSB-YP004462CXX
CSB-EP004462CXX
CSB-BP004462CXX
CSB-MP004462CXX
CSB-EP004462CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytoplasmic tRNA 2-thiolation protein 2 (CBG09667) CSB-YP003313CXX
CSB-EP003313CXX
CSB-BP003313CXX
CSB-MP003313CXX
CSB-EP003313CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flap endonuclease 1 (crn-1) CSB-YP008585CXX
CSB-EP008585CXX
CSB-BP008585CXX
CSB-MP008585CXX
CSB-EP008585CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant F-box/SPRY domain-containing protein 1 (fsn-1) CSB-YP008510CXX
CSB-EP008510CXX
CSB-BP008510CXX
CSB-MP008510CXX
CSB-EP008510CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant U1 small nuclear ribonucleoprotein C (CBG19656) CSB-YP022331CXX
CSB-EP022331CXX
CSB-BP022331CXX
CSB-MP022331CXX
CSB-EP022331CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inosine triphosphate pyrophosphatase (hap-1) CSB-YP011907CXX
CSB-EP011907CXX
CSB-BP011907CXX
CSB-MP011907CXX
CSB-EP011907CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nuclear cap-binding protein subunit 2 (ncbp-2) CSB-YP015521CXX
CSB-EP015521CXX
CSB-BP015521CXX
CSB-MP015521CXX
CSB-EP015521CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus Flap endonuclease 1 (fen) CSB-YP008585NFB
CSB-EP008585NFB
CSB-BP008585NFB
CSB-MP008585NFB
CSB-EP008585NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Signal recognition particle 19 kDa protein (srp19) CSB-YP022674MNQ
CSB-EP022674MNQ
CSB-BP022674MNQ
CSB-MP022674MNQ
CSB-EP022674MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Transaldolase (tal) CSB-YP023112BPS
CSB-EP023112BPS
CSB-BP023112BPS
CSB-MP023112BPS
CSB-EP023112BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans ATP synthase subunit delta (atpH) CSB-YP002355BPS
CSB-EP002355BPS
CSB-BP002355BPS
CSB-MP002355BPS
CSB-EP002355BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Thymidylate kinase (tmk) CSB-YP007226HSK
CSB-EP007226HSK
CSB-BP007226HSK
CSB-MP007226HSK
CSB-EP007226HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Methionyl-tRNA formyltransferase (fmt) CSB-YP015150HSK
CSB-EP015150HSK
CSB-BP015150HSK
CSB-MP015150HSK
CSB-EP015150HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus ATP synthase subunit b (atpF), partial CSB-YP002358PZC1
CSB-EP002358PZC1
CSB-BP002358PZC1
CSB-MP002358PZC1
CSB-EP002358PZC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146PZC
CSB-EP019146PZC
CSB-BP019146PZC
CSB-MP019146PZC
CSB-EP019146PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ferrochelatase (hemH) CSB-YP008579PZC
CSB-EP008579PZC
CSB-BP008579PZC
CSB-MP008579PZC
CSB-EP008579PZC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis tRNA dimethylallyltransferase (miaA) CSB-YP024529PYI
CSB-EP024529PYI
CSB-BP024529PYI
CSB-MP024529PYI
CSB-EP024529PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Petrotoga mobilis Ribosome-recycling factor (frr) CSB-YP014941PYI
CSB-EP014941PYI
CSB-BP014941PYI
CSB-MP014941PYI
CSB-EP014941PYI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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