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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Phosphoglucosamine mutase (glmM) CSB-YP806643BMZ
CSB-EP806643BMZ
CSB-BP806643BMZ
CSB-MP806643BMZ
CSB-EP806643BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae RutC family protein bbp_334 (bbp_334) CSB-YP806644BMZ
CSB-EP806644BMZ
CSB-BP806644BMZ
CSB-MP806644BMZ
CSB-EP806644BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Uncharacterized deoxyribonuclease bbp_325 (bbp_325) CSB-YP806645BMZ
CSB-EP806645BMZ
CSB-BP806645BMZ
CSB-MP806645BMZ
CSB-EP806645BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Ribosomal large subunit pseudouridine synthase C (rluC) CSB-YP806646BMZ
CSB-EP806646BMZ
CSB-BP806646BMZ
CSB-MP806646BMZ
CSB-EP806646BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Flagellar basal-body rod protein flgF (flgF) CSB-YP806647BMZ
CSB-EP806647BMZ
CSB-BP806647BMZ
CSB-MP806647BMZ
CSB-EP806647BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Uncharacterized protein bbp_301 (bbp_301) CSB-YP806648BMZ
CSB-EP806648BMZ
CSB-BP806648BMZ
CSB-MP806648BMZ
CSB-EP806648BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Thioredoxin reductase (trxB) CSB-YP806649BMZ
CSB-EP806649BMZ
CSB-BP806649BMZ
CSB-MP806649BMZ
CSB-EP806649BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Flavodoxin (fldA) CSB-YP806650BMZ
CSB-EP806650BMZ
CSB-BP806650BMZ
CSB-MP806650BMZ
CSB-EP806650BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Malonyl-CoA O-methyltransferase BioC (bioC) CSB-YP806651BMZ
CSB-EP806651BMZ
CSB-BP806651BMZ
CSB-MP806651BMZ
CSB-EP806651BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Uncharacterized acyl-CoA thioester hydrolase bbp_254 (bbp_254) CSB-YP806652BMZ
CSB-EP806652BMZ
CSB-BP806652BMZ
CSB-MP806652BMZ
CSB-EP806652BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae GTPase Era (era) CSB-YP806653BMZ
CSB-EP806653BMZ
CSB-BP806653BMZ
CSB-MP806653BMZ
CSB-EP806653BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 30S ribosomal protein S2 (rpsB) CSB-YP806654BMZ
CSB-EP806654BMZ
CSB-BP806654BMZ
CSB-MP806654BMZ
CSB-EP806654BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Cell division protein FtsZ (ftsZ) CSB-YP806655BMZ
CSB-EP806655BMZ
CSB-BP806655BMZ
CSB-MP806655BMZ
CSB-EP806655BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Penicillin-binding protein 1B (mrcB), partial CSB-YP806656BMZ
CSB-EP806656BMZ
CSB-BP806656BMZ
CSB-MP806656BMZ
CSB-EP806656BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP806657BMZ
CSB-EP806657BMZ
CSB-BP806657BMZ
CSB-MP806657BMZ
CSB-EP806657BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Phosphate acetyltransferase (pta), partial CSB-YP806658BMZ
CSB-EP806658BMZ
CSB-BP806658BMZ
CSB-MP806658BMZ
CSB-EP806658BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Bifunctional protein folC (folC) CSB-YP806659BMZ
CSB-EP806659BMZ
CSB-BP806659BMZ
CSB-MP806659BMZ
CSB-EP806659BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae NADH-quinone oxidoreductase subunit E (nuoE) CSB-YP806660BMZ
CSB-EP806660BMZ
CSB-BP806660BMZ
CSB-MP806660BMZ
CSB-EP806660BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Bis (5'-nucleosyl)-tetraphosphatase, symmetrical (apaH) CSB-YP806661BMZ
CSB-EP806661BMZ
CSB-BP806661BMZ
CSB-MP806661BMZ
CSB-EP806661BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 50S ribosomal protein L20 (rplT) CSB-YP806662BMZ
CSB-EP806662BMZ
CSB-BP806662BMZ
CSB-MP806662BMZ
CSB-EP806662BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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