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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bradyrhizobium japonicum Urocanate hydratase (hutU), partial CSB-YP805735BVW
CSB-EP805735BVW
CSB-BP805735BVW
CSB-MP805735BVW
CSB-EP805735BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum S-adenosylmethionine synthase (metK) CSB-YP805736BVW
CSB-EP805736BVW
CSB-BP805736BVW
CSB-MP805736BVW
CSB-EP805736BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Flagellar hook-basal body complex protein FliE 1 (fliE1) CSB-YP805737BVW
CSB-EP805737BVW
CSB-BP805737BVW
CSB-MP805737BVW
CSB-EP805737BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP805738BVW
CSB-EP805738BVW
CSB-BP805738BVW
CSB-MP805738BVW
CSB-EP805738BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP805739BVW
CSB-EP805739BVW
CSB-BP805739BVW
CSB-MP805739BVW
CSB-EP805739BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 30S ribosomal protein S7 (rpsG) CSB-YP805740BVW
CSB-EP805740BVW
CSB-BP805740BVW
CSB-MP805740BVW
CSB-EP805740BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L2 (rplB) CSB-YP805741BVW
CSB-EP805741BVW
CSB-BP805741BVW
CSB-MP805741BVW
CSB-EP805741BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L14 (rplN) CSB-YP805742BVW
CSB-EP805742BVW
CSB-BP805742BVW
CSB-MP805742BVW
CSB-EP805742BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L15 (rplO) CSB-YP805743BVW
CSB-EP805743BVW
CSB-BP805743BVW
CSB-MP805743BVW
CSB-EP805743BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Pantothenate synthetase 2 (panC2) CSB-YP805744BVW
CSB-EP805744BVW
CSB-BP805744BVW
CSB-MP805744BVW
CSB-EP805744BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP805745BVW
CSB-EP805745BVW
CSB-BP805745BVW
CSB-MP805745BVW
CSB-EP805745BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum N utilization substance protein B homolog (nusB) CSB-YP805746BVW
CSB-EP805746BVW
CSB-BP805746BVW
CSB-MP805746BVW
CSB-EP805746BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L13 (rplM) CSB-YP805747BVW
CSB-EP805747BVW
CSB-BP805747BVW
CSB-MP805747BVW
CSB-EP805747BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP805748BVW
CSB-EP805748BVW
CSB-BP805748BVW
CSB-MP805748BVW
CSB-EP805748BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Triosephosphate isomerase (tpiA) CSB-YP805749BVW
CSB-EP805749BVW
CSB-BP805749BVW
CSB-MP805749BVW
CSB-EP805749BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 5'-nucleotidase surE (surE) CSB-YP805750BVW
CSB-EP805750BVW
CSB-BP805750BVW
CSB-MP805750BVW
CSB-EP805750BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Bifunctional protein GlmU (glmU) CSB-YP805751BVW
CSB-EP805751BVW
CSB-BP805751BVW
CSB-MP805751BVW
CSB-EP805751BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Lipoyl synthase 1 (lipA1) CSB-YP805752BVW
CSB-EP805752BVW
CSB-BP805752BVW
CSB-MP805752BVW
CSB-EP805752BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum C4-dicarboxylate transport protein 3 (dctA3), partial CSB-YP805753BVW
CSB-EP805753BVW
CSB-BP805753BVW
CSB-MP805753BVW
CSB-EP805753BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Aspartate--tRNA ligase (aspS), partial CSB-YP805754BVW
CSB-EP805754BVW
CSB-BP805754BVW
CSB-MP805754BVW
CSB-EP805754BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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