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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lactobacillus plantarum FMN-dependent NADH-azoreductase 1 (azoR1) CSB-YP807090LMS
CSB-EP807090LMS
CSB-BP807090LMS
CSB-MP807090LMS
CSB-EP807090LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Single-stranded DNA-binding protein (ssb) CSB-YP807091LMS
CSB-EP807091LMS
CSB-BP807091LMS
CSB-MP807091LMS
CSB-EP807091LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucosamine-6-phosphate deaminase (nagB) CSB-YP807092CMN
CSB-EP807092CMN
CSB-BP807092CMN
CSB-MP807092CMN
CSB-EP807092CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP807093CMN
CSB-EP807093CMN
CSB-BP807093CMN
CSB-MP807093CMN
CSB-EP807093CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S3 (rpsC) CSB-YP807094CMN
CSB-EP807094CMN
CSB-BP807094CMN
CSB-MP807094CMN
CSB-EP807094CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L18 (rplR) CSB-YP807095CMN
CSB-EP807095CMN
CSB-BP807095CMN
CSB-MP807095CMN
CSB-EP807095CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA pseudouridine synthase A 2 (truA2) CSB-YP807096CMN
CSB-EP807096CMN
CSB-BP807096CMN
CSB-MP807096CMN
CSB-EP807096CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP807097CMN
CSB-EP807097CMN
CSB-BP807097CMN
CSB-MP807097CMN
CSB-EP807097CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Serine/threonine-protein kinase 2 (VPK2) CSB-YP807098VAI
CSB-EP807098VAI
CSB-BP807098VAI
CSB-MP807098VAI
CSB-EP807098VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized RNA methyltransferase CTC_02481 (CTC_02481) CSB-YP807099CMN
CSB-EP807099CMN
CSB-BP807099CMN
CSB-MP807099CMN
CSB-EP807099CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP807100CMN
CSB-EP807100CMN
CSB-BP807100CMN
CSB-MP807100CMN
CSB-EP807100CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trigger factor (tig) CSB-YP807101CMN
CSB-EP807101CMN
CSB-BP807101CMN
CSB-MP807101CMN
CSB-EP807101CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazolonepropionase (hutI) CSB-YP807102CMN
CSB-EP807102CMN
CSB-BP807102CMN
CSB-MP807102CMN
CSB-EP807102CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrodipicolinate synthase (dapA) CSB-YP807103CMN
CSB-EP807103CMN
CSB-BP807103CMN
CSB-MP807103CMN
CSB-EP807103CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillin-binding protein 1A (pbpA), partial CSB-YP807104CMN
CSB-EP807104CMN
CSB-BP807104CMN
CSB-MP807104CMN
CSB-EP807104CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maf-like protein CTC_02076 (CTC_02076) CSB-YP807105CMN
CSB-EP807105CMN
CSB-BP807105CMN
CSB-MP807105CMN
CSB-EP807105CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP807106CMN
CSB-EP807106CMN
CSB-BP807106CMN
CSB-MP807106CMN
CSB-EP807106CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTPase Era (era) CSB-YP807107CMN
CSB-EP807107CMN
CSB-BP807107CMN
CSB-MP807107CMN
CSB-EP807107CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidine--tRNA ligase (hisS) CSB-YP807108CMN
CSB-EP807108CMN
CSB-BP807108CMN
CSB-MP807108CMN
CSB-EP807108CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0324 membrane protein CTC_01844 (CTC_01844), partial CSB-YP807109CMN
CSB-EP807109CMN
CSB-BP807109CMN
CSB-MP807109CMN
CSB-EP807109CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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