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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP807963SMH
CSB-EP807963SMH
CSB-BP807963SMH
CSB-MP807963SMH
CSB-EP807963SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative dihydroorotate dehydrogenase A (fumarate) (pyrD) CSB-YP807964SMH
CSB-EP807964SMH
CSB-BP807964SMH
CSB-MP807964SMH
CSB-EP807964SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP807965SMH
CSB-EP807965SMH
CSB-BP807965SMH
CSB-MP807965SMH
CSB-EP807965SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized RNA methyltransferase gbs0448 (gbs0448) CSB-YP807966SMH
CSB-EP807966SMH
CSB-BP807966SMH
CSB-MP807966SMH
CSB-EP807966SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0398 protein gbs0290 (gbs0290) CSB-YP807967SMH
CSB-EP807967SMH
CSB-BP807967SMH
CSB-MP807967SMH
CSB-EP807967SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant FMN-dependent NADH-azoreductase (azoR) CSB-YP807968SMH
CSB-EP807968SMH
CSB-BP807968SMH
CSB-MP807968SMH
CSB-EP807968SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP807969SMH
CSB-EP807969SMH
CSB-BP807969SMH
CSB-MP807969SMH
CSB-EP807969SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L28 (rpmB) CSB-YP807970SMH
CSB-EP807970SMH
CSB-BP807970SMH
CSB-MP807970SMH
CSB-EP807970SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP807971SMH
CSB-EP807971SMH
CSB-BP807971SMH
CSB-MP807971SMH
CSB-EP807971SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S14 type Z (rpsZ) CSB-YP807972SMH
CSB-EP807972SMH
CSB-BP807972SMH
CSB-MP807972SMH
CSB-EP807972SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L3 (rplC) CSB-YP807973SMH
CSB-EP807973SMH
CSB-BP807973SMH
CSB-MP807973SMH
CSB-EP807973SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptidyl-tRNA hydrolase (pth) CSB-YP807974SMH
CSB-EP807974SMH
CSB-BP807974SMH
CSB-MP807974SMH
CSB-EP807974SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis ATP synthase gamma chain (atpG) CSB-YP807975SGA
CSB-EP807975SGA
CSB-BP807975SGA
CSB-MP807975SGA
CSB-EP807975SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Der GTPase-activating protein YihI (yihI) CSB-YP807976SGA
CSB-EP807976SGA
CSB-BP807976SGA
CSB-MP807976SGA
CSB-EP807976SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Sulfate/thiosulfate import ATP-binding protein CysA 2 (cysA2) CSB-YP807977SGA
CSB-EP807977SGA
CSB-BP807977SGA
CSB-MP807977SGA
CSB-EP807977SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP807978SGA
CSB-EP807978SGA
CSB-BP807978SGA
CSB-MP807978SGA
CSB-EP807978SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Diaminopimelate epimerase (dapF) CSB-YP807979SGA
CSB-EP807979SGA
CSB-BP807979SGA
CSB-MP807979SGA
CSB-EP807979SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis UPF0758 protein SO_4248 (SO_4248) CSB-YP807980SGA
CSB-EP807980SGA
CSB-BP807980SGA
CSB-MP807980SGA
CSB-EP807980SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Glycerol kinase (glpK) CSB-YP807981SGA
CSB-EP807981SGA
CSB-BP807981SGA
CSB-MP807981SGA
CSB-EP807981SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP807982SGA
CSB-EP807982SGA
CSB-BP807982SGA
CSB-MP807982SGA
CSB-EP807982SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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