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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant DNA mismatch repair protein mutL (mutL) CSB-YP808163EGX
CSB-EP808163EGX
CSB-BP808163EGX
CSB-MP808163EGX
CSB-EP808163EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysine--tRNA ligase, heat inducible (lysU) CSB-YP808164EGX
CSB-EP808164EGX
CSB-BP808164EGX
CSB-MP808164EGX
CSB-EP808164EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-coenzyme A synthetase (acs) CSB-YP808165EGX
CSB-EP808165EGX
CSB-BP808165EGX
CSB-MP808165EGX
CSB-EP808165EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0337 protein yjbJ (yjbJ) CSB-YP808166EGX
CSB-EP808166EGX
CSB-BP808166EGX
CSB-MP808166EGX
CSB-EP808166EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal large subunit pseudouridine synthase F (rluF) CSB-YP808167EGX
CSB-EP808167EGX
CSB-BP808167EGX
CSB-MP808167EGX
CSB-EP808167EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylamine--glycine ligase (purD) CSB-YP808168EGX
CSB-EP808168EGX
CSB-BP808168EGX
CSB-MP808168EGX
CSB-EP808168EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate racemase (murI) CSB-YP808169EGX
CSB-EP808169EGX
CSB-BP808169EGX
CSB-MP808169EGX
CSB-EP808169EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-rhamnose-proton symporter (rhaT), partial CSB-YP808170EGX
CSB-EP808170EGX
CSB-BP808170EGX
CSB-MP808170EGX
CSB-EP808170EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein FdhD (fdhD) CSB-YP808171EGX
CSB-EP808171EGX
CSB-BP808171EGX
CSB-MP808171EGX
CSB-EP808171EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sec-independent protein translocase protein TatC (tatC), partial CSB-YP808172EGX1
CSB-EP808172EGX1
CSB-BP808172EGX1
CSB-MP808172EGX1
CSB-EP808172EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylate cyclase (cyaA), partial CSB-YP808173EGX
CSB-EP808173EGX
CSB-BP808173EGX
CSB-MP808173EGX
CSB-EP808173EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ketol-acid reductoisomerase (ilvC) CSB-YP808174EGX
CSB-EP808174EGX
CSB-BP808174EGX
CSB-MP808174EGX
CSB-EP808174EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit a (atpB), partial CSB-YP808175EGX1
CSB-EP808175EGX1
CSB-BP808175EGX1
CSB-MP808175EGX1
CSB-EP808175EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative transport protein YidE (yidE), partial CSB-YP808176EGX
CSB-EP808176EGX
CSB-BP808176EGX
CSB-MP808176EGX
CSB-EP808176EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) CSB-YP808177EGX
CSB-EP808177EGX
CSB-BP808177EGX
CSB-MP808177EGX
CSB-EP808177EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylose import ATP-binding protein XylG (xylG) CSB-YP808178EGX
CSB-EP808178EGX
CSB-BP808178EGX
CSB-MP808178EGX
CSB-EP808178EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional regulator gadW (gadW) CSB-YP808179EGX
CSB-EP808179EGX
CSB-BP808179EGX
CSB-MP808179EGX
CSB-EP808179EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nickel import ATP-binding protein NikD (nikD) CSB-YP808180EGX
CSB-EP808180EGX
CSB-BP808180EGX
CSB-MP808180EGX
CSB-EP808180EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) CSB-YP808181EGX
CSB-EP808181EGX
CSB-BP808181EGX
CSB-MP808181EGX
CSB-EP808181EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionyl-tRNA formyltransferase (fmt) CSB-YP808182EGX
CSB-EP808182EGX
CSB-BP808182EGX
CSB-MP808182EGX
CSB-EP808182EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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