Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chlorobium tepidum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP809213DST
CSB-EP809213DST
CSB-BP809213DST
CSB-MP809213DST
CSB-EP809213DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum ATP synthase subunit delta (atpH) CSB-YP809214DST
CSB-EP809214DST
CSB-BP809214DST
CSB-MP809214DST
CSB-EP809214DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Preprotein translocase subunit SecE (secE), partial CSB-YP809215RMZ
CSB-EP809215RMZ
CSB-BP809215RMZ
CSB-MP809215RMZ
CSB-EP809215RMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL), partial CSB-YP809216BXG
CSB-EP809216BXG
CSB-BP809216BXG
CSB-MP809216BXG
CSB-EP809216BXG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL), partial CSB-YP809217SNB
CSB-EP809217SNB
CSB-BP809217SNB
CSB-MP809217SNB
CSB-EP809217SNB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Nitrogenase-stabilizing/protective protein nifW (nifW) CSB-YP809218RKK
CSB-EP809218RKK
CSB-BP809218RKK
CSB-MP809218RKK
CSB-EP809218RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus necator Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP809219DZQ
CSB-EP809219DZQ
CSB-BP809219DZQ
CSB-MP809219DZQ
CSB-EP809219DZQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL), partial CSB-YP809220OBAQ
CSB-EP809220OBAQ
CSB-BP809220OBAQ
CSB-MP809220OBAQ
CSB-EP809220OBAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus Photosystem II reaction center protein L (psbL), partial CSB-YP809221FPY
CSB-EP809221FPY
CSB-BP809221FPY
CSB-MP809221FPY
CSB-EP809221FPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum L-lactate dehydrogenase (ldh) CSB-YP809222DUT
CSB-EP809222DUT
CSB-BP809222DUT
CSB-MP809222DUT
CSB-EP809222DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP809223XBB
CSB-EP809223XBB
CSB-BP809223XBB
CSB-MP809223XBB
CSB-EP809223XBB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delftia acidovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (bphD) CSB-YP809224DBE
CSB-EP809224DBE
CSB-BP809224DBE
CSB-MP809224DBE
CSB-EP809224DBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit c, sodium ion specific (atpE), partial CSB-YP809225ICAL
CSB-EP809225ICAL
CSB-BP809225ICAL
CSB-MP809225ICAL
CSB-EP809225ICAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP809226DPS1
CSB-EP809226DPS1
CSB-BP809226DPS1
CSB-MP809226DPS1
CSB-EP809226DPS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Tu (tuf) CSB-YP809227RMZ
CSB-EP809227RMZ
CSB-BP809227RMZ
CSB-MP809227RMZ
CSB-EP809227RMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor G (fusA), partial CSB-YP809228RNA
CSB-EP809228RNA
CSB-BP809228RNA
CSB-MP809228RNA
CSB-EP809228RNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant SsrA-binding protein (smpB) CSB-YP809229DPX
CSB-EP809229DPX
CSB-BP809229DPX
CSB-MP809229DPX
CSB-EP809229DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP809230THN
CSB-EP809230THN
CSB-BP809230THN
CSB-MP809230THN
CSB-EP809230THN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Potassium-transporting ATPase B chain (kdpB), partial CSB-YP809231ELW1
CSB-EP809231ELW1
CSB-BP809231ELW1
CSB-MP809231ELW1
CSB-EP809231ELW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus Tyrosine recombinase XerC (xerC) CSB-YP809232FPY
CSB-EP809232FPY
CSB-BP809232FPY
CSB-MP809232FPY
CSB-EP809232FPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>