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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon (cheB1) CSB-YP809911LOS
CSB-EP809911LOS
CSB-BP809911LOS
CSB-MP809911LOS
CSB-EP809911LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai 2-oxoglutarate dehydrogenase E1 component (sucA), partial CSB-YP809912LOS
CSB-EP809912LOS
CSB-BP809912LOS
CSB-MP809912LOS
CSB-EP809912LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP809913LOS
CSB-EP809913LOS
CSB-BP809913LOS
CSB-MP809913LOS
CSB-EP809913LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai 50S ribosomal protein L31 (rpmE) CSB-YP809914LOS
CSB-EP809914LOS
CSB-BP809914LOS
CSB-MP809914LOS
CSB-EP809914LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Translation initiation factor IF-2 (infB), partial CSB-YP809915LOS
CSB-EP809915LOS
CSB-BP809915LOS
CSB-MP809915LOS
CSB-EP809915LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP809916LOS1
CSB-EP809916LOS1
CSB-BP809916LOS1
CSB-MP809916LOS1
CSB-EP809916LOS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai 50S ribosomal protein L21 (rplU) CSB-YP809917LOS
CSB-EP809917LOS
CSB-BP809917LOS
CSB-MP809917LOS
CSB-EP809917LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Type III pantothenate kinase (coaX) CSB-YP809918LOS
CSB-EP809918LOS
CSB-BP809918LOS
CSB-MP809918LOS
CSB-EP809918LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Shikimate dehydrogenase (aroE) CSB-YP809919LOS
CSB-EP809919LOS
CSB-BP809919LOS
CSB-MP809919LOS
CSB-EP809919LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Glycine cleavage system H protein (gcvH) CSB-YP809920LOS
CSB-EP809920LOS
CSB-BP809920LOS
CSB-MP809920LOS
CSB-EP809920LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Elongation factor G (fusA), partial CSB-YP809921LOS
CSB-EP809921LOS
CSB-BP809921LOS
CSB-MP809921LOS
CSB-EP809921LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai 50S ribosomal protein L34 (rpmH) CSB-YP809922LOS
CSB-EP809922LOS
CSB-BP809922LOS
CSB-MP809922LOS
CSB-EP809922LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai Peptide chain release factor 1 (prfA) CSB-YP809923LOS
CSB-EP809923LOS
CSB-BP809923LOS
CSB-MP809923LOS
CSB-EP809923LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP809924EGX
CSB-EP809924EGX
CSB-BP809924EGX
CSB-MP809924EGX
CSB-EP809924EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Valine--tRNA ligase (valS), partial CSB-YP809925EGX
CSB-EP809925EGX
CSB-BP809925EGX
CSB-MP809925EGX
CSB-EP809925EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-keto-L-gulonate-6-phosphate decarboxylase UlaD (ulaD) CSB-YP809926EGX
CSB-EP809926EGX
CSB-BP809926EGX
CSB-MP809926EGX
CSB-EP809926EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable L-ascorbate-6-phosphate lactonase ulaG (ulaG) CSB-YP809927EGX
CSB-EP809927EGX
CSB-BP809927EGX
CSB-MP809927EGX
CSB-EP809927EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0401 protein c5157 (c5157) CSB-YP809928EGX
CSB-EP809928EGX
CSB-BP809928EGX
CSB-MP809928EGX
CSB-EP809928EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein ytcA (ytcA), partial CSB-YP809929EGX1
CSB-EP809929EGX1
CSB-BP809929EGX1
CSB-MP809929EGX1
CSB-EP809929EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maltose transport system permease protein malF (malF), partial CSB-YP809930EGX
CSB-EP809930EGX
CSB-BP809930EGX
CSB-MP809930EGX
CSB-EP809930EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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