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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Yersinia pestis bv. Antiqua Triosephosphate isomerase (tpiA) CSB-YP024102YAM
CSB-EP024102YAM
CSB-BP024102YAM
CSB-MP024102YAM
CSB-EP024102YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Xaa-Pro dipeptidase (pepQ) CSB-YP017784YAM
CSB-EP017784YAM
CSB-BP017784YAM
CSB-MP017784YAM
CSB-EP017784YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Serine hydroxymethyltransferase (glyA) CSB-YP021274YAM
CSB-EP021274YAM
CSB-BP021274YAM
CSB-MP021274YAM
CSB-EP021274YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii NADH-ubiquinone oxidoreductase chain 2 (ND2), partial CSB-YP015077DIS
CSB-EP015077DIS
CSB-BP015077DIS
CSB-MP015077DIS
CSB-EP015077DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii ATP synthase subunit a (ATP6), partial CSB-YP015070DIS1
CSB-EP015070DIS1
CSB-BP015070DIS1
CSB-MP015070DIS1
CSB-EP015070DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein Muted homolog (muted) CSB-YP015244XBF
CSB-EP015244XBF
CSB-BP015244XBF
CSB-MP015244XBF
CSB-EP015244XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis 40S ribosomal protein SA (30792) CSB-YP020485MOD
CSB-EP020485MOD
CSB-BP020485MOD
CSB-MP020485MOD
CSB-EP020485MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis GDP-fucose transporter 1 (slc35c1), partial CSB-YP021592MOD
CSB-EP021592MOD
CSB-BP021592MOD
CSB-MP021592MOD
CSB-EP021592MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis Eukaryotic translation initiation factor 3 subunit H (9558) CSB-YP007537MOD
CSB-EP007537MOD
CSB-BP007537MOD
CSB-MP007537MOD
CSB-EP007537MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis Eukaryotic translation initiation factor 3 subunit L (39212), partial CSB-YP007541MOD
CSB-EP007541MOD
CSB-BP007541MOD
CSB-MP007541MOD
CSB-EP007541MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis tRNA dimethylallyltransferase (miaA) CSB-YP024529BON
CSB-EP024529BON
CSB-BP024529BON
CSB-MP024529BON
CSB-EP024529BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis ATP synthase subunit beta (atpD) CSB-YP002350BON
CSB-EP002350BON
CSB-BP002350BON
CSB-MP002350BON
CSB-EP002350BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Serine hydroxymethyltransferase (glyA) CSB-YP021274BON
CSB-EP021274BON
CSB-BP021274BON
CSB-MP021274BON
CSB-EP021274BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens ATP synthase subunit b (atpF), partial CSB-YP002358MNY1
CSB-EP002358MNY1
CSB-BP002358MNY1
CSB-MP002358MNY1
CSB-EP002358MNY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens tRNA pseudouridine synthase A (truA) CSB-YP019086MNY
CSB-EP019086MNY
CSB-BP019086MNY
CSB-MP019086MNY
CSB-EP019086MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Ribosome-recycling factor (frr) CSB-YP014941MNY
CSB-EP014941MNY
CSB-BP014941MNY
CSB-MP014941MNY
CSB-EP014941MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Acetyl-coenzyme A synthetase (acsA), partial CSB-YP001203MNY
CSB-EP001203MNY
CSB-BP001203MNY
CSB-MP001203MNY
CSB-EP001203MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Elongation factor Ts (tsf) CSB-YP025124RIH
CSB-EP025124RIH
CSB-BP025124RIH
CSB-MP025124RIH
CSB-EP025124RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum ATP synthase subunit b (atpF), partial CSB-YP002358RIH1
CSB-EP002358RIH1
CSB-BP002358RIH1
CSB-MP002358RIH1
CSB-EP002358RIH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Methionyl-tRNA formyltransferase (fmt) CSB-YP015150RIH
CSB-EP015150RIH
CSB-BP015150RIH
CSB-MP015150RIH
CSB-EP015150RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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