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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila erecta Diacylglycerol kinase eta (GG14241), partial CSB-YP006837DLK
CSB-EP006837DLK
CSB-BP006837DLK
CSB-MP006837DLK
CSB-EP006837DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Nuclear cap-binding protein subunit 2 (Cbp20) CSB-YP015521DLK
CSB-EP015521DLK
CSB-BP015521DLK
CSB-MP015521DLK
CSB-EP015521DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Eukaryotic translation initiation factor 3 subunit A (eIF3-S10), partial CSB-YP007543DLK
CSB-EP007543DLK
CSB-BP007543DLK
CSB-MP007543DLK
CSB-EP007543DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein kibra (Kibra), partial CSB-YP026163DLK
CSB-EP026163DLK
CSB-BP026163DLK
CSB-MP026163DLK
CSB-EP026163DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma arthritidis Elongation factor Ts (tsf) CSB-YP025124MWD
CSB-EP025124MWD
CSB-BP025124MWD
CSB-MP025124MWD
CSB-EP025124MWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Acetyl-coenzyme A synthetase (acsA), partial CSB-YP001203RKL
CSB-EP001203RKL
CSB-BP001203RKL
CSB-MP001203RKL
CSB-EP001203RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Acyl carrier protein (acpP) CSB-YP015636RLV
CSB-EP015636RLV
CSB-BP015636RLV
CSB-MP015636RLV
CSB-EP015636RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Adenosylhomocysteinase (ahcY) CSB-YP001474RLV
CSB-EP001474RLV
CSB-BP001474RLV
CSB-MP001474RLV
CSB-EP001474RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Elongation factor Ts (tsf) CSB-YP025124DSQ
CSB-EP025124DSQ
CSB-BP025124DSQ
CSB-MP025124DSQ
CSB-EP025124DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum ATP synthase subunit delta (atpH) CSB-YP002355DSQ
CSB-EP002355DSQ
CSB-BP002355DSQ
CSB-MP002355DSQ
CSB-EP002355DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Translation initiation factor IF-2 (infB), partial CSB-YP015164DSQ
CSB-EP015164DSQ
CSB-BP015164DSQ
CSB-MP015164DSQ
CSB-EP015164DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Elongation factor G (fusA), partial CSB-YP009373DSQ
CSB-EP009373DSQ
CSB-BP009373DSQ
CSB-MP009373DSQ
CSB-EP009373DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Malate dehydrogenase (mdh) CSB-YP013623DSY
CSB-EP013623DSY
CSB-BP013623DSY
CSB-MP013623DSY
CSB-EP013623DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium ATP synthase subunit beta (atpD) CSB-YP002350DSY
CSB-EP002350DSY
CSB-BP002350DSY
CSB-MP002350DSY
CSB-EP002350DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium ATP synthase subunit a (atpB), partial CSB-YP015070DSY1
CSB-EP015070DSY1
CSB-BP015070DSY1
CSB-MP015070DSY1
CSB-EP015070DSY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Translation initiation factor IF-2 (infB), partial CSB-YP015164DZS
CSB-EP015164DZS
CSB-BP015164DZS
CSB-MP015164DZS
CSB-EP015164DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Malate dehydrogenase (mdh) CSB-YP013623DZS
CSB-EP013623DZS
CSB-BP013623DZS
CSB-MP013623DZS
CSB-EP013623DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146DZS
CSB-EP019146DZS
CSB-BP019146DZS
CSB-MP019146DZS
CSB-EP019146DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis ATP synthase subunit beta (atpD) CSB-YP002350DZS
CSB-EP002350DZS
CSB-BP002350DZS
CSB-MP002350DZS
CSB-EP002350DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichoplax adhaerens Ubiquitin-fold modifier-conjugating enzyme 1 (TRIADDRAFT_35934) CSB-YP025558TQD
CSB-EP025558TQD
CSB-BP025558TQD
CSB-MP025558TQD
CSB-EP025558TQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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