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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB Ferrochelatase (hemH) CSB-YP008579YAW
CSB-EP008579YAW
CSB-BP008579YAW
CSB-MP008579YAW
CSB-EP008579YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB ATP synthase subunit b (atpF), partial CSB-YP002358YAW1
CSB-EP002358YAW1
CSB-BP002358YAW1
CSB-MP002358YAW1
CSB-EP002358YAW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582YAW
CSB-EP009582YAW
CSB-BP009582YAW
CSB-MP009582YAW
CSB-EP009582YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhinolophus ferrumequinum Tumor necrosis factor alpha-induced protein 8-like protein 2 (TNFAIP8L2) CSB-YP023962RKD
CSB-EP023962RKD
CSB-BP023962RKD
CSB-MP023962RKD
CSB-EP023962RKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhinolophus ferrumequinum WD repeat-containing protein 76 (WDR76), partial CSB-YP026064RKD
CSB-EP026064RKD
CSB-BP026064RKD
CSB-MP026064RKD
CSB-EP026064RKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Translation initiation factor IF-3 (infC) CSB-YP015166EXZ
CSB-EP015166EXZ
CSB-BP015166EXZ
CSB-MP015166EXZ
CSB-EP015166EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Plexin-B2 (Plxnb2), partial CSB-YP018223MO
CSB-EP018223MO
CSB-BP018223MO
CSB-MP018223MO
CSB-EP018223MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia hermsii Triosephosphate isomerase (tpiA) CSB-YP024102BUJ
CSB-EP024102BUJ
CSB-BP024102BUJ
CSB-MP024102BUJ
CSB-EP024102BUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum GTPase Era (era) CSB-YP007759TPS
CSB-EP007759TPS
CSB-BP007759TPS
CSB-MP007759TPS
CSB-EP007759TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum tRNA dimethylallyltransferase (miaA) CSB-YP024529TPS
CSB-EP024529TPS
CSB-BP024529TPS
CSB-MP024529TPS
CSB-EP024529TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum Ribonuclease 3 (rnc) CSB-YP019809TPS
CSB-EP019809TPS
CSB-BP019809TPS
CSB-MP019809TPS
CSB-EP019809TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus tRNA dimethylallyltransferase (miaA) CSB-YP024529BWR
CSB-EP024529BWR
CSB-BP024529BWR
CSB-MP024529BWR
CSB-EP024529BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus ATP synthase subunit delta (atpH) CSB-YP002355BWR
CSB-EP002355BWR
CSB-BP002355BWR
CSB-MP002355BWR
CSB-EP002355BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Peptide chain release factor 1 (prfA) CSB-YP015197BWR
CSB-EP015197BWR
CSB-BP015197BWR
CSB-MP015197BWR
CSB-EP015197BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917BWR
CSB-EP022917BWR
CSB-BP022917BWR
CSB-MP022917BWR
CSB-EP022917BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717BWR
CSB-EP009717BWR
CSB-BP009717BWR
CSB-MP009717BWR
CSB-EP009717BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Ferrochelatase (hemH) CSB-YP008579BWR
CSB-EP008579BWR
CSB-BP008579BWR
CSB-MP008579BWR
CSB-EP008579BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica ATP synthase subunit delta (atpH) CSB-YP002355FFF
CSB-EP002355FFF
CSB-BP002355FFF
CSB-MP002355FFF
CSB-EP002355FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Peptide chain release factor 1 (prfA) CSB-YP015197FFF
CSB-EP015197FFF
CSB-BP015197FFF
CSB-MP015197FFF
CSB-EP015197FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Nucleoside diphosphate kinase (ndk) CSB-YP015889FFF
CSB-EP015889FFF
CSB-BP015889FFF
CSB-MP015889FFF
CSB-EP015889FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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