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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. mediasiatica Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146FFF
CSB-EP019146FFF
CSB-BP019146FFF
CSB-MP019146FFF
CSB-EP019146FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582XBC
CSB-EP009582XBC
CSB-BP009582XBC
CSB-MP009582XBC
CSB-EP009582XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Porphobilinogen deaminase (hemC) CSB-YP010524XBC
CSB-EP010524XBC
CSB-BP010524XBC
CSB-MP010524XBC
CSB-EP010524XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Acyl carrier protein (acpP) CSB-YP015636XBC
CSB-EP015636XBC
CSB-BP015636XBC
CSB-MP015636XBC
CSB-EP015636XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Peptide chain release factor 1 (prfA) CSB-YP015197BXU
CSB-EP015197BXU
CSB-BP015197BXU
CSB-MP015197BXU
CSB-EP015197BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Porphobilinogen deaminase (hemC) CSB-YP010524BXU
CSB-EP010524BXU
CSB-BP010524BXU
CSB-MP010524BXU
CSB-EP010524BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717BXU
CSB-EP009717BXU
CSB-BP009717BXU
CSB-MP009717BXU
CSB-EP009717BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Ribosome-recycling factor (frr) CSB-YP014941BXU
CSB-EP014941BXU
CSB-BP014941BXU
CSB-MP014941BXU
CSB-EP014941BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans ATP synthase subunit delta (atpH) CSB-YP002355BXU
CSB-EP002355BXU
CSB-BP002355BXU
CSB-MP002355BXU
CSB-EP002355BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Malate dehydrogenase (mdh) CSB-YP013623BXU
CSB-EP013623BXU
CSB-BP013623BXU
CSB-MP013623BXU
CSB-EP013623BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum tRNA dimethylallyltransferase (miaA) CSB-YP024529DUG
CSB-EP024529DUG
CSB-BP024529DUG
CSB-MP024529DUG
CSB-EP024529DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum tRNA pseudouridine synthase A (truA) CSB-YP019086DUG
CSB-EP019086DUG
CSB-BP019086DUG
CSB-MP019086DUG
CSB-EP019086DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP synthase subunit beta (atpD) CSB-YP002350DUG
CSB-EP002350DUG
CSB-BP002350DUG
CSB-MP002350DUG
CSB-EP002350DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 ATP synthase subunit beta (atpD) CSB-YP002350SYZ
CSB-EP002350SYZ
CSB-BP002350SYZ
CSB-MP002350SYZ
CSB-EP002350SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582SYZ
CSB-EP009582SYZ
CSB-BP009582SYZ
CSB-MP009582SYZ
CSB-EP009582SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 GTPase Era (era) CSB-YP007759SYZ
CSB-EP007759SYZ
CSB-BP007759SYZ
CSB-MP007759SYZ
CSB-EP007759SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Peptide deformylase (def) CSB-YP017707SYZ
CSB-EP017707SYZ
CSB-BP017707SYZ
CSB-MP017707SYZ
CSB-EP017707SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146SYZ
CSB-EP019146SYZ
CSB-BP019146SYZ
CSB-MP019146SYZ
CSB-EP019146SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Acyl carrier protein (acpP) CSB-YP015636SYZ
CSB-EP015636SYZ
CSB-BP015636SYZ
CSB-MP015636SYZ
CSB-EP015636SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Adenosylhomocysteinase (ahcY) CSB-YP001474RJI
CSB-EP001474RJI
CSB-BP001474RJI
CSB-MP001474RJI
CSB-EP001474RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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