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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila virilis Adenylyltransferase and sulfurtransferase MOCS3 (GJ21670) CSB-YP014707DMP
CSB-EP014707DMP
CSB-BP014707DMP
CSB-MP014707DMP
CSB-EP014707DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Protein kibra (Kibra), partial CSB-YP026163DMP
CSB-EP026163DMP
CSB-BP026163DMP
CSB-MP026163DMP
CSB-EP026163DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Nuclear cap-binding protein subunit 1 (Cbp80), partial CSB-YP015520DMP
CSB-EP015520DMP
CSB-BP015520DMP
CSB-MP015520DMP
CSB-EP015520DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis DNA repair and recombination protein RAD54-like (okr), partial CSB-YP019271DMP
CSB-EP019271DMP
CSB-BP019271DMP
CSB-MP019271DMP
CSB-EP019271DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Protein kintoun (Ppi20), partial CSB-YP003132DMQ
CSB-EP003132DMQ
CSB-BP003132DMQ
CSB-MP003132DMQ
CSB-EP003132DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni F-box/SPRY domain-containing protein 1 (Fsn) CSB-YP008510DMQ
CSB-EP008510DMQ
CSB-BP008510DMQ
CSB-MP008510DMQ
CSB-EP008510DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Adenylyltransferase and sulfurtransferase MOCS3 (GK18675) CSB-YP014707DMQ
CSB-EP014707DMQ
CSB-BP014707DMQ
CSB-MP014707DMQ
CSB-EP014707DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Methylthioribose-1-phosphate isomerase (GK22419) CSB-YP014799DMQ
CSB-EP014799DMQ
CSB-BP014799DMQ
CSB-MP014799DMQ
CSB-EP014799DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Nucleolar protein 6 (Mat89Ba), partial CSB-YP015924DMQ
CSB-EP015924DMQ
CSB-BP015924DMQ
CSB-MP015924DMQ
CSB-EP015924DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni 40S ribosomal protein SA (sta) CSB-YP020485DMQ
CSB-EP020485DMQ
CSB-BP020485DMQ
CSB-MP020485DMQ
CSB-EP020485DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Eukaryotic translation initiation factor 3 subunit I (Trip1) CSB-YP007538DMR
CSB-EP007538DMR
CSB-BP007538DMR
CSB-MP007538DMR
CSB-EP007538DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Cytoplasmic tRNA 2-thiolation protein 1 (GE22576) CSB-YP002426DMR
CSB-EP002426DMR
CSB-BP002426DMR
CSB-MP002426DMR
CSB-EP002426DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Flap endonuclease 1 (Fen1) CSB-YP008585DMR
CSB-EP008585DMR
CSB-BP008585DMR
CSB-MP008585DMR
CSB-EP008585DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Protein N-terminal glutamine amidohydrolase (tun) CSB-YP026083DMR
CSB-EP026083DMR
CSB-BP026083DMR
CSB-MP026083DMR
CSB-EP026083DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Diacylglycerol kinase eta (GE24946), partial CSB-YP006837DMR
CSB-EP006837DMR
CSB-BP006837DMR
CSB-MP006837DMR
CSB-EP006837DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Ubiquitin-like modifier-activating enzyme 5 (GE15940) CSB-YP025415DMR
CSB-EP025415DMR
CSB-BP025415DMR
CSB-MP025415DMR
CSB-EP025415DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Serrate RNA effector molecule homolog (Ars2), partial CSB-YP022696DMJ
CSB-EP022696DMJ
CSB-BP022696DMJ
CSB-MP022696DMJ
CSB-EP022696DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Protein N-terminal glutamine amidohydrolase (tun) CSB-YP026083DMJ
CSB-EP026083DMJ
CSB-BP026083DMJ
CSB-MP026083DMJ
CSB-EP026083DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Serine/threonine-protein kinase PLK4 (SAK), partial CSB-YP018196DMJ
CSB-EP018196DMJ
CSB-BP018196DMJ
CSB-MP018196DMJ
CSB-EP018196DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Probable methylthioribulose-1-phosphate dehydratase (GD17142) CSB-YP001906DMJ
CSB-EP001906DMJ
CSB-BP001906DMJ
CSB-MP001906DMJ
CSB-EP001906DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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