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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila ananassae Eukaryotic translation initiation factor 3 subunit I (Trip1) CSB-YP007538DKY
CSB-EP007538DKY
CSB-BP007538DKY
CSB-MP007538DKY
CSB-EP007538DKY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Zinc finger CCCH-type with G patch domain-containing protein (GG10072), partial CSB-YP026480DLK
CSB-EP026480DLK
CSB-BP026480DLK
CSB-MP026480DLK
CSB-EP026480DLK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Ubiquitin carboxyl-terminal hydrolase 36 (Usp36), partial CSB-YP025727DLK
CSB-EP025727DLK
CSB-BP025727DLK
CSB-MP025727DLK
CSB-EP025727DLK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Cytoplasmic tRNA 2-thiolation protein 2 (GG21627) CSB-YP003313DLK
CSB-EP003313DLK
CSB-BP003313DLK
CSB-MP003313DLK
CSB-EP003313DLK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Flap endonuclease 1 (Fen1) CSB-YP008585DLK
CSB-EP008585DLK
CSB-BP008585DLK
CSB-MP008585DLK
CSB-EP008585DLK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Ubiquitin-like modifier-activating enzyme 5 (GG18422) CSB-YP025415DLK
CSB-EP025415DLK
CSB-BP025415DLK
CSB-MP025415DLK
CSB-EP025415DLK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Enolase-phosphatase E1 (GG11122) CSB-YP007674DLK
CSB-EP007674DLK
CSB-BP007674DLK
CSB-MP007674DLK
CSB-EP007674DLK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta 40S ribosomal protein SA (sta) CSB-YP020485DLK
CSB-EP020485DLK
CSB-BP020485DLK
CSB-MP020485DLK
CSB-EP020485DLK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus ATP synthase subunit delta (atpH) CSB-YP002355DQY
CSB-EP002355DQY
CSB-BP002355DQY
CSB-MP002355DQY
CSB-EP002355DQY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma arthritidis ATP synthase subunit delta (atpH) CSB-YP002355MWD
CSB-EP002355MWD
CSB-BP002355MWD
CSB-MP002355MWD
CSB-EP002355MWD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma arthritidis Elongation factor Tu (tuf) CSB-YP025342MWD
CSB-EP025342MWD
CSB-BP025342MWD
CSB-MP025342MWD
CSB-EP025342MWD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli ATP synthase subunit alpha (atpA), partial CSB-YP002344RKL
CSB-EP002344RKL
CSB-BP002344RKL
CSB-MP002344RKL
CSB-EP002344RKL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Elongation factor Ts (tsf) CSB-YP025124RKL
CSB-EP025124RKL
CSB-BP025124RKL
CSB-MP025124RKL
CSB-EP025124RKL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Lipoyl synthase (lipA) CSB-YP012927RKL
CSB-EP012927RKL
CSB-BP012927RKL
CSB-MP012927RKL
CSB-EP012927RKL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris ATP synthase subunit delta (atpH) CSB-YP002355RLV
CSB-EP002355RLV
CSB-BP002355RLV
CSB-MP002355RLV
CSB-EP002355RLV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917DSQ
CSB-EP022917DSQ
CSB-BP022917DSQ
CSB-MP022917DSQ
CSB-EP022917DSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Malate dehydrogenase (mdh) CSB-YP013623DSQ
CSB-EP013623DSQ
CSB-BP013623DSQ
CSB-MP013623DSQ
CSB-EP013623DSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali Elongation factor Tu (tuf) CSB-YP025342EVH
CSB-EP025342EVH
CSB-BP025342EVH
CSB-MP025342EVH
CSB-EP025342EVH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali tRNA pseudouridine synthase A (truA) CSB-YP019086EVH
CSB-EP019086EVH
CSB-BP019086EVH
CSB-MP019086EVH
CSB-EP019086EVH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali tRNA dimethylallyltransferase (miaA) CSB-YP024529EVH
CSB-EP024529EVH
CSB-BP024529EVH
CSB-MP024529EVH
CSB-EP024529EVH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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