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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cellvibrio japonicus tRNA dimethylallyltransferase (miaA) CSB-YP024529DQY
CSB-EP024529DQY
CSB-BP024529DQY
CSB-MP024529DQY
CSB-EP024529DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus ATP synthase subunit alpha (atpA), partial CSB-YP002344DQY
CSB-EP002344DQY
CSB-BP002344DQY
CSB-MP002344DQY
CSB-EP002344DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma arthritidis Serine hydroxymethyltransferase (glyA) CSB-YP021274MWD
CSB-EP021274MWD
CSB-BP021274MWD
CSB-MP021274MWD
CSB-EP021274MWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP009493RKL
CSB-EP009493RKL
CSB-BP009493RKL
CSB-MP009493RKL
CSB-EP009493RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli ATP synthase subunit delta (atpH) CSB-YP002355RKL
CSB-EP002355RKL
CSB-BP002355RKL
CSB-MP002355RKL
CSB-EP002355RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Acyl carrier protein (acpP) CSB-YP015636RKL
CSB-EP015636RKL
CSB-BP015636RKL
CSB-MP015636RKL
CSB-EP015636RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Glucokinase (glk) CSB-YP009319RKL
CSB-EP009319RKL
CSB-BP009319RKL
CSB-MP009319RKL
CSB-EP009319RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582RKL
CSB-EP009582RKL
CSB-BP009582RKL
CSB-MP009582RKL
CSB-EP009582RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Peptide deformylase (def) CSB-YP017707RLV
CSB-EP017707RLV
CSB-BP017707RLV
CSB-MP017707RLV
CSB-EP017707RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Ribosome-recycling factor (frr) CSB-YP014941DSQ
CSB-EP014941DSQ
CSB-BP014941DSQ
CSB-MP014941DSQ
CSB-EP014941DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Adenosylhomocysteinase (ahcY) CSB-YP001474DSQ
CSB-EP001474DSQ
CSB-BP001474DSQ
CSB-MP001474DSQ
CSB-EP001474DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium tRNA dimethylallyltransferase (miaA) CSB-YP024529DSY
CSB-EP024529DSY
CSB-BP024529DSY
CSB-MP024529DSY
CSB-EP024529DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582DSY
CSB-EP009582DSY
CSB-BP009582DSY
CSB-MP009582DSY
CSB-EP009582DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali Elongation factor Ts (tsf) CSB-YP025124EVH
CSB-EP025124EVH
CSB-BP025124EVH
CSB-MP025124EVH
CSB-EP025124EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Acetyl-coenzyme A synthetase (acsA), partial CSB-YP001203DZS
CSB-EP001203DZS
CSB-BP001203DZS
CSB-MP001203DZS
CSB-EP001203DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis ATP synthase subunit b (atpF), partial CSB-YP002358DZS1
CSB-EP002358DZS1
CSB-BP002358DZS1
CSB-MP002358DZS1
CSB-EP002358DZS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichoplax adhaerens Alpha-tubulin N-acetyltransferase (TRIADDRAFT_53139) CSB-YP004059TQD
CSB-EP004059TQD
CSB-BP004059TQD
CSB-MP004059TQD
CSB-EP004059TQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichoplax adhaerens Flap endonuclease 1 (FEN1) CSB-YP008585TQD
CSB-EP008585TQD
CSB-BP008585TQD
CSB-MP008585TQD
CSB-EP008585TQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichoplax adhaerens 40S ribosomal protein S3a (TRIADDRAFT_36460) CSB-YP020444TQD
CSB-EP020444TQD
CSB-BP020444TQD
CSB-MP020444TQD
CSB-EP020444TQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei ATP synthase subunit beta (atpD) CSB-YP002350LMJ
CSB-EP002350LMJ
CSB-BP002350LMJ
CSB-MP002350LMJ
CSB-EP002350LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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