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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Drosophila sechellia Ubiquitin-conjugating enzyme E2 S (GM13299) CSB-YP025477DMG
CSB-EP025477DMG
CSB-BP025477DMG
CSB-MP025477DMG
CSB-EP025477DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Phosphotriesterase-related protein (GH16075) CSB-YP018966DLL
CSB-EP018966DLL
CSB-BP018966DLL
CSB-MP018966DLL
CSB-EP018966DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Adenylyltransferase and sulfurtransferase MOCS3 (GH10959) CSB-YP014707DLL
CSB-EP014707DLL
CSB-BP014707DLL
CSB-MP014707DLL
CSB-EP014707DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi KRR1 small subunit processome component homolog (dbe) CSB-YP012502DLL
CSB-EP012502DLL
CSB-BP012502DLL
CSB-MP012502DLL
CSB-EP012502DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila grimshawi Eukaryotic translation initiation factor 3 subunit K (GH22679) CSB-YP007540DLL
CSB-EP007540DLL
CSB-BP007540DLL
CSB-MP007540DLL
CSB-EP007540DLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Phosphotriesterase-related protein (GI24003) CSB-YP018966DLY
CSB-EP018966DLY
CSB-BP018966DLY
CSB-MP018966DLY
CSB-EP018966DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Nucleolar protein 6 (Mat89Ba), partial CSB-YP015924DLY
CSB-EP015924DLY
CSB-BP015924DLY
CSB-MP015924DLY
CSB-EP015924DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Eukaryotic translation initiation factor 3 subunit H (eIF-3p40) CSB-YP007537DLY
CSB-EP007537DLY
CSB-BP007537DLY
CSB-MP007537DLY
CSB-EP007537DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Adenylyltransferase and sulfurtransferase MOCS3 (GI10453) CSB-YP014707DLY
CSB-EP014707DLY
CSB-BP014707DLY
CSB-MP014707DLY
CSB-EP014707DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Serrate RNA effector molecule homolog (Ars2), partial CSB-YP022696DLY
CSB-EP022696DLY
CSB-BP022696DLY
CSB-MP022696DLY
CSB-EP022696DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Eukaryotic translation initiation factor 3 subunit B (eIF3-S9), partial CSB-YP007530DLY
CSB-EP007530DLY
CSB-BP007530DLY
CSB-MP007530DLY
CSB-EP007530DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Protein arginine N-methyltransferase 7 (Art7), partial CSB-YP018736DLY
CSB-EP018736DLY
CSB-BP018736DLY
CSB-MP018736DLY
CSB-EP018736DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Eukaryotic translation initiation factor 3 subunit J (Adam) CSB-YP007539DLY
CSB-EP007539DLY
CSB-BP007539DLY
CSB-MP007539DLY
CSB-EP007539DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Ubiquitin-related modifier 1 homolog (GJ12350) CSB-YP025676DMP
CSB-EP025676DMP
CSB-BP025676DMP
CSB-MP025676DMP
CSB-EP025676DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Ubiquitin carboxyl-terminal hydrolase 36 (Usp36), partial CSB-YP025727DMP
CSB-EP025727DMP
CSB-BP025727DMP
CSB-MP025727DMP
CSB-EP025727DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Ubiquitin-fold modifier-conjugating enzyme 1 (GJ20088) CSB-YP025558DMP
CSB-EP025558DMP
CSB-BP025558DMP
CSB-MP025558DMP
CSB-EP025558DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis F-box/SPRY domain-containing protein 1 (Fsn) CSB-YP008510DMP
CSB-EP008510DMP
CSB-BP008510DMP
CSB-MP008510DMP
CSB-EP008510DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Protein SMG8 (GJ17645), partial CSB-YP003375DMP
CSB-EP003375DMP
CSB-BP003375DMP
CSB-MP003375DMP
CSB-EP003375DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Enolase-phosphatase E1 (GJ11016) CSB-YP007674DMP
CSB-EP007674DMP
CSB-BP007674DMP
CSB-MP007674DMP
CSB-EP007674DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Lysine-specific demethylase NO66 (GJ17031), partial CSB-YP003168DMP
CSB-EP003168DMP
CSB-BP003168DMP
CSB-MP003168DMP
CSB-EP003168DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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