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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xylella fastidiosa tRNA pseudouridine synthase A (truA) CSB-YP019086XBN
CSB-EP019086XBN
CSB-BP019086XBN
CSB-MP019086XBN
CSB-EP019086XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa S-adenosylmethionine decarboxylase proenzyme (speD) CSB-YP001658XBN
CSB-EP001658XBN
CSB-BP001658XBN
CSB-MP001658XBN
CSB-EP001658XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica ATP synthase subunit a (atpB), partial CSB-YP015070BSP1
CSB-EP015070BSP1
CSB-BP015070BSP1
CSB-MP015070BSP1
CSB-EP015070BSP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beijerinckia indica subsp. indica Elongation factor G (fusA), partial CSB-YP009373BSP
CSB-EP009373BSP
CSB-BP009373BSP
CSB-MP009373BSP
CSB-EP009373BSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae ATP synthase subunit beta (atpD) CSB-YP002350FMZ
CSB-EP002350FMZ
CSB-BP002350FMZ
CSB-MP002350FMZ
CSB-EP002350FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Ribosome-recycling factor (frr) CSB-YP014941NHQ
CSB-EP014941NHQ
CSB-BP014941NHQ
CSB-MP014941NHQ
CSB-EP014941NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Nucleoside diphosphate kinase (ndk) CSB-YP015889NHQ
CSB-EP015889NHQ
CSB-BP015889NHQ
CSB-MP015889NHQ
CSB-EP015889NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme ATP synthase subunit alpha (atpA), partial CSB-YP002344NHQ
CSB-EP002344NHQ
CSB-BP002344NHQ
CSB-MP002344NHQ
CSB-EP002344NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Glucokinase (glk) CSB-YP009319NHQ
CSB-EP009319NHQ
CSB-BP009319NHQ
CSB-MP009319NHQ
CSB-EP009319NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Transaldolase (tal) CSB-YP023112NHQ
CSB-EP023112NHQ
CSB-BP023112NHQ
CSB-MP023112NHQ
CSB-EP023112NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP009493NHQ
CSB-EP009493NHQ
CSB-BP009493NHQ
CSB-MP009493NHQ
CSB-EP009493NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717NHQ
CSB-EP009717NHQ
CSB-BP009717NHQ
CSB-MP009717NHQ
CSB-EP009717NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799NHQ
CSB-EP014799NHQ
CSB-BP014799NHQ
CSB-MP014799NHQ
CSB-EP014799NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Translation initiation factor IF-2 (infB), partial CSB-YP015164NHQ
CSB-EP015164NHQ
CSB-BP015164NHQ
CSB-MP015164NHQ
CSB-EP015164NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Acetyl-coenzyme A synthetase (acsA), partial CSB-YP001203BXT
CSB-EP001203BXT
CSB-BP001203BXT
CSB-MP001203BXT
CSB-EP001203BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Ferrochelatase (hemH) CSB-YP008579BXT
CSB-EP008579BXT
CSB-BP008579BXT
CSB-MP008579BXT
CSB-EP008579BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Thymidylate kinase (tmk) CSB-YP007226BXT
CSB-EP007226BXT
CSB-BP007226BXT
CSB-MP007226BXT
CSB-EP007226BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Adenosylhomocysteinase (ahcY) CSB-YP001474BXT
CSB-EP001474BXT
CSB-BP001474BXT
CSB-MP001474BXT
CSB-EP001474BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Glycine cleavage system H protein (gcvH) CSB-YP009335BXT
CSB-EP009335BXT
CSB-BP009335BXT
CSB-MP009335BXT
CSB-EP009335BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phymatum Methionyl-tRNA formyltransferase (fmt) CSB-YP015150BXT
CSB-EP015150BXT
CSB-BP015150BXT
CSB-MP015150BXT
CSB-EP015150BXT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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