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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Helicobacter pylori tRNA dimethylallyltransferase (miaA) CSB-YP024529HUZ
CSB-EP024529HUZ
CSB-BP024529HUZ
CSB-MP024529HUZ
CSB-EP024529HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Thymidylate kinase (tmk) CSB-YP007226HUZ
CSB-EP007226HUZ
CSB-BP007226HUZ
CSB-MP007226HUZ
CSB-EP007226HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033DUF
CSB-EP018033DUF
CSB-BP018033DUF
CSB-MP018033DUF
CSB-EP018033DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Argininosuccinate synthase (argG) CSB-YP002234DUF
CSB-EP002234DUF
CSB-BP002234DUF
CSB-MP002234DUF
CSB-EP002234DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum ATP synthase subunit alpha (atpA), partial CSB-YP002344DUF
CSB-EP002344DUF
CSB-BP002344DUF
CSB-MP002344DUF
CSB-EP002344DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum 6-phosphofructokinase (pfkA) CSB-YP017822DUF
CSB-EP017822DUF
CSB-BP017822DUF
CSB-MP017822DUF
CSB-EP017822DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Peptide deformylase (def) CSB-YP017707DUF
CSB-EP017707DUF
CSB-BP017707DUF
CSB-MP017707DUF
CSB-EP017707DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Glycine cleavage system H protein (gcvH) CSB-YP009335ENB
CSB-EP009335ENB
CSB-BP009335ENB
CSB-MP009335ENB
CSB-EP009335ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Xaa-Pro dipeptidase (pepQ) CSB-YP017784ENB
CSB-EP017784ENB
CSB-BP017784ENB
CSB-MP017784ENB
CSB-EP017784ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Beta-galactosidase (lacZ), partial CSB-YP009476ENB
CSB-EP009476ENB
CSB-BP009476ENB
CSB-MP009476ENB
CSB-EP009476ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrenophora tritici-repentis Structure-specific endonuclease subunit slx1 (slx1) CSB-YP009442FHT
CSB-EP009442FHT
CSB-BP009442FHT
CSB-MP009442FHT
CSB-EP009442FHT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lycium barbarum Non-specific lipid-transfer protein CSB-YP020856LRN
CSB-EP020856LRN
CSB-BP020856LRN
CSB-MP020856LRN
CSB-EP020856LRN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Elongation factor G (fusA), partial CSB-YP009373WBO
CSB-EP009373WBO
CSB-BP009373WBO
CSB-MP009373WBO
CSB-EP009373WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Ferrochelatase (hemH) CSB-YP008579WBO
CSB-EP008579WBO
CSB-BP008579WBO
CSB-MP008579WBO
CSB-EP008579WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens tRNA dimethylallyltransferase (miaA) CSB-YP024529WBO
CSB-EP024529WBO
CSB-BP024529WBO
CSB-MP024529WBO
CSB-EP024529WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Lipoyl synthase (lipA) CSB-YP012927WBO
CSB-EP012927WBO
CSB-BP012927WBO
CSB-MP012927WBO
CSB-EP012927WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582WBO
CSB-EP009582WBO
CSB-BP009582WBO
CSB-MP009582WBO
CSB-EP009582WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia pipientis subsp. Culex pipiens Triosephosphate isomerase (tpiA) CSB-YP024102WBO
CSB-EP024102WBO
CSB-BP024102WBO
CSB-MP024102WBO
CSB-EP024102WBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein Spindly (ccdc99), partial CSB-YP004768XBF
CSB-EP004768XBF
CSB-BP004768XBF
CSB-MP004768XBF
CSB-EP004768XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifidobacterium longum Thymidylate kinase (tmk) CSB-YP007226BTA
CSB-EP007226BTA
CSB-BP007226BTA
CSB-MP007226BTA
CSB-EP007226BTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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