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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Takifugu rubripes Leptin receptor gene-related protein (leprot), partial CSB-YP012875TKY1
CSB-EP012875TKY1
CSB-BP012875TKY1
CSB-MP012875TKY1
CSB-EP012875TKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Peptide chain release factor 1 (prfA) CSB-YP015197BXS
CSB-EP015197BXS
CSB-BP015197BXS
CSB-MP015197BXS
CSB-EP015197BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Translation initiation factor IF-2 (infB), partial CSB-YP015164BXS
CSB-EP015164BXS
CSB-BP015164BXS
CSB-MP015164BXS
CSB-EP015164BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia tRNA dimethylallyltransferase (miaA) CSB-YP024529BXS
CSB-EP024529BXS
CSB-BP024529BXS
CSB-MP024529BXS
CSB-EP024529BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia ATP synthase subunit beta (atpD) CSB-YP002350BXS
CSB-EP002350BXS
CSB-BP002350BXS
CSB-MP002350BXS
CSB-EP002350BXS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917EYZ
CSB-EP022917EYZ
CSB-BP022917EYZ
CSB-MP022917EYZ
CSB-EP022917EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis NAD-dependent malic enzyme (maeA), partial CSB-YP013633EYZ
CSB-EP013633EYZ
CSB-BP013633EYZ
CSB-MP013633EYZ
CSB-EP013633EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Serine hydroxymethyltransferase (glyA) CSB-YP021274EYZ
CSB-EP021274EYZ
CSB-BP021274EYZ
CSB-MP021274EYZ
CSB-EP021274EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Ferrochelatase (hemH) CSB-YP008579EYZ
CSB-EP008579EYZ
CSB-BP008579EYZ
CSB-MP008579EYZ
CSB-EP008579EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Elongation factor Ts (tsf) CSB-YP025124EYZ
CSB-EP025124EYZ
CSB-BP025124EYZ
CSB-MP025124EYZ
CSB-EP025124EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Apoptosis-inducing factor 2 (aifm2), partial CSB-YP001493XBF1
CSB-EP001493XBF1
CSB-BP001493XBF1
CSB-MP001493XBF1
CSB-EP001493XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Oxidation resistance protein 1 (oxr1), partial CSB-YP017312XBE
CSB-EP017312XBE
CSB-BP017312XBE
CSB-MP017312XBE
CSB-EP017312XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis F-box/SPRY domain-containing protein 1 (fbxo45) CSB-YP008510XBF
CSB-EP008510XBF
CSB-BP008510XBF
CSB-MP008510XBF
CSB-EP008510XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat High mobility group nucleosome-binding domain-containing protein 5 (Hmgn5) CSB-YP016099RA
CSB-EP016099RA
CSB-BP016099RA
CSB-MP016099RA
CSB-EP016099RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Flap endonuclease 1 (FEN1) CSB-YP008585ZAX
CSB-EP008585ZAX
CSB-BP008585ZAX
CSB-MP008585ZAX
CSB-EP008585ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Kinetochore protein Spc25 (Spc25) CSB-YP022513DMB
CSB-EP022513DMB
CSB-BP022513DMB
CSB-MP022513DMB
CSB-EP022513DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Nuclear cap-binding protein subunit 1 (Cbp80), partial CSB-YP015520DMB
CSB-EP015520DMB
CSB-BP015520DMB
CSB-MP015520DMB
CSB-EP015520DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Serrate RNA effector molecule homolog (Ars2), partial CSB-YP022696DMB
CSB-EP022696DMB
CSB-BP022696DMB
CSB-MP022696DMB
CSB-EP022696DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Eukaryotic translation initiation factor 3 subunit C (eIF3-S8), partial CSB-YP007531DMG
CSB-EP007531DMG
CSB-BP007531DMG
CSB-MP007531DMG
CSB-EP007531DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila sechellia Cytoplasmic tRNA 2-thiolation protein 1 (GM20632) CSB-YP002426DMG
CSB-EP002426DMG
CSB-BP002426DMG
CSB-MP002426DMG
CSB-EP002426DMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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