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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila virilis Eukaryotic translation initiation factor 3 subunit J (Adam) CSB-YP007539DMP
CSB-EP007539DMP
CSB-BP007539DMP
CSB-MP007539DMP
CSB-EP007539DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis KRR1 small subunit processome component homolog (dbe) CSB-YP012502DMP
CSB-EP012502DMP
CSB-BP012502DMP
CSB-MP012502DMP
CSB-EP012502DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Cytoplasmic tRNA 2-thiolation protein 2 (GJ18095) CSB-YP003313DMP
CSB-EP003313DMP
CSB-BP003313DMP
CSB-MP003313DMP
CSB-EP003313DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Eukaryotic translation initiation factor 3 subunit B (eIF3-S9), partial CSB-YP007530DMQ
CSB-EP007530DMQ
CSB-BP007530DMQ
CSB-MP007530DMQ
CSB-EP007530DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Flap endonuclease 1 (Fen1) CSB-YP008585DMQ
CSB-EP008585DMQ
CSB-BP008585DMQ
CSB-MP008585DMQ
CSB-EP008585DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Eukaryotic translation initiation factor 3 subunit L (GK20774), partial CSB-YP007541DMQ
CSB-EP007541DMQ
CSB-BP007541DMQ
CSB-MP007541DMQ
CSB-EP007541DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Probable cytosolic iron-sulfur protein assembly protein Ciao1 (Ciao1) CSB-YP005423DMQ
CSB-EP005423DMQ
CSB-BP005423DMQ
CSB-MP005423DMQ
CSB-EP005423DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni KRR1 small subunit processome component homolog (dbe) CSB-YP012502DMQ
CSB-EP012502DMQ
CSB-BP012502DMQ
CSB-MP012502DMQ
CSB-EP012502DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Enolase-phosphatase E1 (GK14286) CSB-YP007674DMQ
CSB-EP007674DMQ
CSB-BP007674DMQ
CSB-MP007674DMQ
CSB-EP007674DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni MAU2 chromatid cohesion factor homolog (GK13302), partial CSB-YP012213DMQ
CSB-EP012213DMQ
CSB-BP012213DMQ
CSB-MP012213DMQ
CSB-EP012213DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Serrate RNA effector molecule homolog (Ars2), partial CSB-YP022696DMR
CSB-EP022696DMR
CSB-BP022696DMR
CSB-MP022696DMR
CSB-EP022696DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Eukaryotic translation initiation factor 3 subunit B (eIF3-S9), partial CSB-YP007530DMR
CSB-EP007530DMR
CSB-BP007530DMR
CSB-MP007530DMR
CSB-EP007530DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Vacuolar ATPase assembly integral membrane protein VMA21 (GE19686), partial CSB-YP025866DMR1
CSB-EP025866DMR1
CSB-BP025866DMR1
CSB-MP025866DMR1
CSB-EP025866DMR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Ubiquitin carboxyl-terminal hydrolase 36 (Usp36), partial CSB-YP025727DMR
CSB-EP025727DMR
CSB-BP025727DMR
CSB-MP025727DMR
CSB-EP025727DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Eukaryotic translation initiation factor 3 subunit A (eIF3-S10), partial CSB-YP007543DMR
CSB-EP007543DMR
CSB-BP007543DMR
CSB-MP007543DMR
CSB-EP007543DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Phosphotriesterase-related protein (GE25944) CSB-YP018966DMR
CSB-EP018966DMR
CSB-BP018966DMR
CSB-MP018966DMR
CSB-EP018966DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans F-box/SPRY domain-containing protein 1 (Fsn) CSB-YP008510DMJ
CSB-EP008510DMJ
CSB-BP008510DMJ
CSB-MP008510DMJ
CSB-EP008510DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Probable cytosolic iron-sulfur protein assembly protein Ciao1 (Ciao1) CSB-YP005423DMJ
CSB-EP005423DMJ
CSB-BP005423DMJ
CSB-MP005423DMJ
CSB-EP005423DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Eukaryotic translation initiation factor 3 subunit J (Adam) CSB-YP007539DMJ
CSB-EP007539DMJ
CSB-BP007539DMJ
CSB-MP007539DMJ
CSB-EP007539DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Molybdopterin synthase catalytic subunit (GD18232) CSB-YP014706DMJ
CSB-EP014706DMJ
CSB-BP014706DMJ
CSB-MP014706DMJ
CSB-EP014706DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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