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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chloroherpeton thalassium Peptide chain release factor 1 (prfA) CSB-YP015197DSY
CSB-EP015197DSY
CSB-BP015197DSY
CSB-MP015197DSY
CSB-EP015197DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium ATP synthase subunit delta (atpH) CSB-YP002355DSY
CSB-EP002355DSY
CSB-BP002355DSY
CSB-MP002355DSY
CSB-EP002355DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis tRNA pseudouridine synthase A (truA) CSB-YP019086DZS
CSB-EP019086DZS
CSB-BP019086DZS
CSB-MP019086DZS
CSB-EP019086DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Transaldolase (tal) CSB-YP023112DZS
CSB-EP023112DZS
CSB-BP023112DZS
CSB-MP023112DZS
CSB-EP023112DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717DZS
CSB-EP009717DZS
CSB-BP009717DZS
CSB-MP009717DZS
CSB-EP009717DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichoplax adhaerens tRNA-splicing ligase RtcB homolog (TRIADDRAFT_18395), partial CSB-YP003810TQD
CSB-EP003810TQD
CSB-BP003810TQD
CSB-MP003810TQD
CSB-EP003810TQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Nuclear receptor subfamily 1 group D member 1, partial CSB-YP016043SH
CSB-EP016043SH
CSB-BP016043SH
CSB-MP016043SH
CSB-EP016043SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Galactokinase (galK) CSB-YP009198LMJ
CSB-EP009198LMJ
CSB-BP009198LMJ
CSB-MP009198LMJ
CSB-EP009198LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Triosephosphate isomerase (tpiA) CSB-YP024102LMJ
CSB-EP024102LMJ
CSB-BP024102LMJ
CSB-MP024102LMJ
CSB-EP024102LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Peptide chain release factor 1 (prfA) CSB-YP015197LMJ
CSB-EP015197LMJ
CSB-BP015197LMJ
CSB-MP015197LMJ
CSB-EP015197LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Translation initiation factor IF-2 (infB), partial CSB-YP015164LMJ
CSB-EP015164LMJ
CSB-BP015164LMJ
CSB-MP015164LMJ
CSB-EP015164LMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Myeloid differentiation primary response protein MyD88 (MYD88) CSB-YP015282EXP
CSB-EP015282EXP
CSB-BP015282EXP
CSB-MP015282EXP
CSB-EP015282EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Cardiolipin synthase (cls), partial CSB-YP005985EYZ1
CSB-EP005985EYZ1
CSB-BP005985EYZ1
CSB-MP005985EYZ1
CSB-EP005985EYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis ATP synthase subunit beta (atpD) CSB-YP002350EYZ
CSB-EP002350EYZ
CSB-BP002350EYZ
CSB-MP002350EYZ
CSB-EP002350EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Adenosine monophosphate-protein transferase FICD (ficd), partial CSB-YP008672XBF1
CSB-EP008672XBF1
CSB-BP008672XBF1
CSB-MP008672XBF1
CSB-EP008672XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Ragulator complex protein LAMTOR3 (lamtor3) CSB-YP013477XBF
CSB-EP013477XBF
CSB-BP013477XBF
CSB-MP013477XBF
CSB-EP013477XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Pescadillo homolog (GL19215), partial CSB-YP017791DMB
CSB-EP017791DMB
CSB-BP017791DMB
CSB-MP017791DMB
CSB-EP017791DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Protein kintoun (Ppi20), partial CSB-YP003132DMB
CSB-EP003132DMB
CSB-BP003132DMB
CSB-MP003132DMB
CSB-EP003132DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis DNA repair and recombination protein RAD54-like (okr), partial CSB-YP019271DMB
CSB-EP019271DMB
CSB-BP019271DMB
CSB-MP019271DMB
CSB-EP019271DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila persimilis Cytoplasmic tRNA 2-thiolation protein 2 (GL21098) CSB-YP003313DMB
CSB-EP003313DMB
CSB-BP003313DMB
CSB-MP003313DMB
CSB-EP003313DMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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