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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Caulobacter crescentus Elongation factor G (fusA), partial CSB-YP009373DQP
CSB-EP009373DQP
CSB-BP009373DQP
CSB-MP009373DQP
CSB-EP009373DQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter chlorophenolicus ATP synthase subunit beta (atpD) CSB-YP002350DOG
CSB-EP002350DOG
CSB-BP002350DOG
CSB-MP002350DOG
CSB-EP002350DOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter chlorophenolicus Translation initiation factor IF-2 (infB), partial CSB-YP015164DOG
CSB-EP015164DOG
CSB-BP015164DOG
CSB-MP015164DOG
CSB-EP015164DOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. tRNA dimethylallyltransferase (miaA) CSB-YP024529EPV
CSB-EP024529EPV
CSB-BP024529EPV
CSB-MP024529EPV
CSB-EP024529EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Glycine cleavage system H protein (gcvH) CSB-YP009335EPV
CSB-EP009335EPV
CSB-BP009335EPV
CSB-MP009335EPV
CSB-EP009335EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Elongation factor Tu (tuf) CSB-YP025342EPV
CSB-EP025342EPV
CSB-BP025342EPV
CSB-MP025342EPV
CSB-EP025342EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Porphobilinogen deaminase (hemC) CSB-YP010524DUL
CSB-EP010524DUL
CSB-BP010524DUL
CSB-MP010524DUL
CSB-EP010524DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum ATP synthase subunit delta (atpH) CSB-YP002355DUL
CSB-EP002355DUL
CSB-BP002355DUL
CSB-MP002355DUL
CSB-EP002355DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Translation initiation factor IF-2 (infB), partial CSB-YP015164DUL
CSB-EP015164DUL
CSB-BP015164DUL
CSB-MP015164DUL
CSB-EP015164DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium nodulans Malate dehydrogenase (mdh) CSB-YP013623MTB
CSB-EP013623MTB
CSB-BP013623MTB
CSB-MP013623MTB
CSB-EP013623MTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium nodulans Ribosome-recycling factor (frr) CSB-YP014941MTB
CSB-EP014941MTB
CSB-BP014941MTB
CSB-MP014941MTB
CSB-EP014941MTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium nodulans Adenosylhomocysteinase (ahcY) CSB-YP001474MTB
CSB-EP001474MTB
CSB-BP001474MTB
CSB-MP001474MTB
CSB-EP001474MTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium nodulans Peptide chain release factor 1 (prfA) CSB-YP015197MTB
CSB-EP015197MTB
CSB-BP015197MTB
CSB-MP015197MTB
CSB-EP015197MTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Elongation factor Ts (tsf) CSB-YP025124EZY
CSB-EP025124EZY
CSB-BP025124EZY
CSB-MP025124EZY
CSB-EP025124EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799EZY
CSB-EP014799EZY
CSB-BP014799EZY
CSB-MP014799EZY
CSB-EP014799EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Ferrochelatase (hemH) CSB-YP008579VFH
CSB-EP008579VFH
CSB-BP008579VFH
CSB-MP008579VFH
CSB-EP008579VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Cysteine desulfurase (iscS) CSB-YP015769VFH
CSB-EP015769VFH
CSB-BP015769VFH
CSB-MP015769VFH
CSB-EP015769VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus tRNA pseudouridine synthase A (truA) CSB-YP019086VFH
CSB-EP019086VFH
CSB-BP019086VFH
CSB-MP019086VFH
CSB-EP019086VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Catenin alpha-2 (Ctnna2), partial CSB-YP006165DIL
CSB-EP006165DIL
CSB-BP006165DIL
CSB-MP006165DIL
CSB-EP006165DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Histone-lysine N-methyltransferase setd3 (setd3), partial CSB-YP021104XBF
CSB-EP021104XBF
CSB-BP021104XBF
CSB-MP021104XBF
CSB-EP021104XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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