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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Desulfatibacillum alkenivorans Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917DJI
CSB-EP022917DJI
CSB-BP022917DJI
CSB-MP022917DJI
CSB-EP022917DJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfitobacterium hafniense Porphobilinogen deaminase (hemC) CSB-YP010524DJJ
CSB-EP010524DJJ
CSB-BP010524DJJ
CSB-MP010524DJJ
CSB-EP010524DJJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aggregans ATP synthase subunit beta (atpD) CSB-YP002350DSV
CSB-EP002350DSV
CSB-BP002350DSV
CSB-MP002350DSV
CSB-EP002350DSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aggregans Elongation factor G (fusA), partial CSB-YP009373DSV
CSB-EP009373DSV
CSB-BP009373DSV
CSB-MP009373DSV
CSB-EP009373DSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aggregans Lipoyl synthase (lipA) CSB-YP012927DSV
CSB-EP012927DSV
CSB-BP012927DSV
CSB-MP012927DSV
CSB-EP012927DSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosphaerula palustris Protein pelota homolog (pelA) CSB-YP017778MSP
CSB-EP017778MSP
CSB-BP017778MSP
CSB-MP017778MSP
CSB-EP017778MSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thioalkalivibrio sp. tRNA dimethylallyltransferase (miaA) CSB-YP024529TNX
CSB-EP024529TNX
CSB-BP024529TNX
CSB-MP024529TNX
CSB-EP024529TNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thioalkalivibrio sp. Translation initiation factor IF-2 (infB), partial CSB-YP015164TNX
CSB-EP015164TNX
CSB-BP015164TNX
CSB-MP015164TNX
CSB-EP015164TNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thioalkalivibrio sp. ATP synthase subunit alpha (atpA), partial CSB-YP002344TNX
CSB-EP002344TNX
CSB-BP002344TNX
CSB-MP002344TNX
CSB-EP002344TNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thioalkalivibrio sp. Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799TNX
CSB-EP014799TNX
CSB-BP014799TNX
CSB-MP014799TNX
CSB-EP014799TNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thioalkalivibrio sp. Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146TNX
CSB-EP019146TNX
CSB-BP019146TNX
CSB-MP019146TNX
CSB-EP019146TNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Argininosuccinate synthase (argG) CSB-YP002234DQP
CSB-EP002234DQP
CSB-BP002234DQP
CSB-MP002234DQP
CSB-EP002234DQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus tRNA pseudouridine synthase A (truA) CSB-YP019086DQP
CSB-EP019086DQP
CSB-BP019086DQP
CSB-MP019086DQP
CSB-EP019086DQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Glycine cleavage system H protein (gcvH) CSB-YP009335DQP
CSB-EP009335DQP
CSB-BP009335DQP
CSB-MP009335DQP
CSB-EP009335DQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter chlorophenolicus ATP synthase subunit a (atpB), partial CSB-YP015070DOG1
CSB-EP015070DOG1
CSB-BP015070DOG1
CSB-MP015070DOG1
CSB-EP015070DOG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter chlorophenolicus DNA ligase 1 (ligA1), partial CSB-YP012930DOG
CSB-EP012930DOG
CSB-BP012930DOG
CSB-MP012930DOG
CSB-EP012930DOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter chlorophenolicus Elongation factor Ts (tsf) CSB-YP025124DOG
CSB-EP025124DOG
CSB-BP025124DOG
CSB-MP025124DOG
CSB-EP025124DOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter chlorophenolicus tRNA dimethylallyltransferase (miaA) CSB-YP024529DOG
CSB-EP024529DOG
CSB-BP024529DOG
CSB-MP024529DOG
CSB-EP024529DOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Ribosome-recycling factor (frr) CSB-YP014941EPV
CSB-EP014941EPV
CSB-BP014941EPV
CSB-MP014941EPV
CSB-EP014941EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824EPV
CSB-EP005824EPV
CSB-BP005824EPV
CSB-MP005824EPV
CSB-EP005824EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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